IL-1R1 蛋白, Human (HEK293, His)

IL-1R1 (CD121a) belongs to IL-1 receptor, and binds to IL-1α, IL-1β, IL-1Ra and IL-38 with comparable affinities (K_d: 0.1-1 nM)^[1]. IL-1R1 regulates the transcription of multiple proinflammatory cytokines and mediates immune and inflammatory responses, including the activation of NF-κB and MAPK pathway^[4]. IL-1R1 Protein, Human (HEK293, His) is a recombinant human extracellular region of IL-1R1 (L18-T332) with a C-Terminal 6*His tag, which is produced in HEK293 cells.

IL-1R1 (CD121a) 是 IL-1 受体的成员，是 IL-1α、IL-1β、IL-1Ra 和 IL-38 的受体，从而介导 IL-1 依赖性激活^{[1]< /sup>。IL-1R1 在特定的内皮细胞、心室细胞、星形胶质细胞和神经元中表达[2]}。
不同物种的 IL-1R1 氨基酸序列不同。人 IL-1R1 与小鼠和大鼠的氨基酸序列一致性 <70%。
IL-1R1 通过与 IL-1R3 (IL-1RAcP) 形成异二聚体来与 IL-1 结合。异二聚体负责通过 GTP 水解进行信号转导，然后是 JNK 和 p38 MAP 激酶^[3]。与 IL-1 结合后，IL-1R1 启动下游信号级联（MAPK p38 和 NF-κB 通路）^[4]。IL-1R1 信号传导诱导人类 Th17 细胞分化和定型，这对于自身免疫性疾病的发展至关重要^[4]。
IL-1R1 介导免疫和炎症反应。IL-1R1介导NF-κB、MAPK通路的激活。

Measured by its ability to inhibit IL-1 beta -dependent proliferation in CTLL-2 cells. Under the action of 30 pg/mL recombinant human IL-1β. The ED₅₀ for this effect is 1.286 μg/mL, corresponding to a specific activity is 7.776×10^2 units/mg.

• 
  Measured by its ability to inhibit IL-1 beta -dependent proliferation in CTLL-2 cells. Under the action of 30 pg/mL recombinant human IL-1β, The ED₅₀ for this effect is 1.286 μg/mL, corresponding to a specific activity is 7.776×10² units/mg.

Human

HEK293

C-6*His

P14778/NP_000868.1 (L18-T332)

3554  [NCBI]

LEADKCKEREEKIILVSSANEIDVRPCPLNPNEHKGTITWYKDDSKTPVSTEQASRIHQHKEKLWFVPAKVEDSGHYYCVVRNSSYCLRIKISAKFVENEPNLCYNAQAIFKQKLPVAGDGGLVCPYMEFFKNENNELPKLQWYKDCKPLLLDNIHFSGVKDRLIVMNVAEKHRGNYTCHASYTYLGKQYPITRVIEFITLEENKPTRPVIVSPANETMEVDLGSQIQLICNVTGQLSDIAYWKWNGSVIDEDDPVLGEDYYSVENPANKRRSTLITVLNISEIESRFYKHPFTCFAKNTHGIDAAYIQLIYPVT

45-62 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.The protein migrates as 45-62 kDa under reducing condition (SDS-PAGE) due to glycosylation.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, Normally 5% trehalose is added as protectant before lyophilization or 20 mM PB, 150 mM NaCl, 5%-8% trehalose, mannitol and 0.01% Tween 80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Diana Boraschi, et al. The family of the interleukin-1 receptors. Immunol Rev. 2018 Jan;281(1):197-232.  [Content Brief]

  [2]. Daniel P Nemeth, et al. Modulation of Neural Networks by Interleukin-1. Brain Plast. 2021 Aug 23;7(1):17-32.  [Content Brief]

  [3]. Naoe Kaneko, et al. The role of interleukin-1 in general pathology. Inflamm Regen. 2019 Jun 6;39:12.  [Content Brief]

  [4]. Yonggang Sha, et al. A role of IL-1R1 signaling in the differentiation of Th17 cells and the development of autoimmune diseases. Self Nonself. 2011 Jan;2(1):35-42.  [Content Brief]