IL-1R2 蛋白, Human (HEK293, His)

IL-1R2 is a competitive endogenous inhibitor of IL-1 signaling. IL-1R2 serves as a decoy receptor and competes with IL-1R1 for IL-1, it can also form a complex with IL-1RAP once it binds IL-1. IL-1R2 is implicated in various IL-1-mediated inflammatory diseases^[1]. IL-1R2 Protein, Human (HEK293, His) is a recombinant human IL-1R2 protein with a His tag at the C-terminus and is expressed in HEK293 cells. It consists of 330 amino acids (F14-E343).

IL-1R2 是非信号传导 2 型白细胞介素 1 受体，天然存在于中性粒细胞、B 细胞、单核细胞和巨噬细胞中。IL-1R2 也在乳腺癌和结肠癌细胞中表达。IL-1R2 在人类调节性 T 细胞 (Treg) 中快速上调。人IL-1R2可被切割成两种形式：膜形式（14-398个aa）和可溶形式（14-？aa）。
人IL-1R2的氨基酸序列相似性较低（约60%）小鼠和大鼠 IL-1R2。
IL-1R2 含有截短的胞质结构域，并且缺乏 Toll-IL-1 受体 (TIR) 区域，使其无法跨膜信号传导。IL-1R2 是 IL-1 信号传导的内源性抑制剂。功能性 IL-1 信号传导需要 IL-1R1 和 IL-1RAP 的 IL-1 依赖性募集。IL-1R2 作为诱饵受体，可以与 IL-1R1 竞争 IL-1。一旦IL-1R2与IL-1结合，IL-1R2也可以与IL-1RAP形成复合物，从而防止IL-1RAP与IL-1R1异二聚化。通过这两种方式，IL-1R2 阻断 IL-1 信号传导。此外，可溶性 IL-1R2 以高亲和力募集可溶性 IL-1RAP，而不影响对 IL-1RA 的亲和力。
IL-1R2 抑制 IL-1 信号传导，并与多种 IL-1 介导的炎症性疾病有关，例如关节炎、糖尿病、痛风等^[1][2][3]。

IL-1R2 but not IL-1R1 or IL-1RAcP is sensitive to PMA-induced proteolytic activity and ectodomain shedding of IL-1R2 is dependent on ADAM17^[5].

Human sIL-1R2 (5 or 25 μg/kg; i.p.; daily for 24 days) decreases the development of endometrial implants and inflammation in mice^[4].

Immobilized Human IL-1R2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC₅₀ of 15.1ng/ml determined by ELISA.

Human

HEK293

C-His

P27930/NP_004624.1 (F14-E343)

7850  [NCBI]

FTLQPAAHTGAARSCRFRGRHYKREFRLEGEPVALRCPQVPYWLWASVSPRINLTWHKNDSARTVPGEEETRMWAQDGALWLLPALQEDSGTYVCTTRNASYCDKMSIELRVFENTDAFLPFISYPQILTLSTSGVLVCPDLSEFTRDKTDVKIQWYKDSLLLDKDNEKFLSVRGTTHLLVHDVALEDAGYYRCVLTFAHEGQQYNITRSIELRIKKKKEETIPVIISPLKTISASLGSRLTIPCKVFLGTGTPLTTMLWWTANDTHIESAYPGGRVTEGPRQEYSENNENYIEVPLIFDPVTREDLHMDFKCVVHNTLSFQTLRTTVKE

Approximately 50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. PetersVA,etal.IL-1receptor2(IL-1R2)anditsroleinimmuneregulation.BrainBehavImmun.2013Aug;32:1-8.  [Content Brief]

  [2]. Molgora M, et al. Tuning inflammation and immunity by the negative regulators IL-1R2 and IL-1R8. Immunol Rev. 2018 Jan;281(1):233-247.  [Content Brief]

  [3]. Lin J, et al. Cardiomyocyte IL-1R2 protects heart from ischemia/reperfusion injury by attenuating IL-17RA-mediated cardiomyocyte apoptosis. Cell Death Dis. 2022 Jan 27;13(1):90.  [Content Brief]

  [4]. Khoufache K, et al. Soluble human IL-1 receptor type 2 inhibits ectopic endometrial tissue implantation and growth: identification of a novel potential target for endometriosis treatment. Am J Pathol. 2012 Oct;181(4):1197-205.  [Content Brief]

  [5]. Uchikawa S, et al. ADAM17 regulates IL-1 signaling by selectively releasing IL-1 receptor type 2 from the cell surface. Cytokine. 2015 Feb;71(2):238-45.  [Content Brief]