IL-2 蛋白, Mouse (HEK293, His-Avi)

IL-2 Protein is usually produces by antigen-activated CD4+ T cells, CD8+ T cells, NK cells and NKT cells. IL-2 Protein is involved in signaling pathways including JAK/STAT, inosine phosphate 3-kinase /PI3K, and mitogen-activated protein kinase /MAPK. IL-2 Protein, Mouse (HEK293, His-Avi) is the recombinant mouse-derived IL-2 protein, expressed by HEK293 , with N-His, N-Avi labeled tag. The total length of IL-2 Protein, Mouse (HEK293, His-Avi) is 149 a.a., with molecular weight of 27-30 kDa.

IL-2 是一种受体细胞因子，由活化的 CD4 阳性辅助性 T 细胞产生，通过激活JAK/STAT、磷酸肌苷-3-激酶/PI3K和丝裂原活化蛋白激酶/MAPK 等多种途径发挥作用。IL-2 可结合由高亲和三聚体IL-2R (IL2RA/CD25, IL2RB/CD122和IL2RG/CD132)或低亲和二聚体IL-2R (IL2RB和IL2RG)组成的受体复合物。IL-2 可增加 NK 细胞的细胞溶解活性。促进活化 B 细胞的强增殖和免疫球蛋白的产生。IL-2 参与效应 T 细胞亚群的分化和稳态，包括 Th1、Th2、Th17 以及记忆 CD8 阳性 T 细胞。IL-2 的合成在 mRNA 水平上受到 TCR 和 CD28 信号的严格调控。可介导激活诱导的细胞死亡 (AICD) 过程。IL-2 可用于癌症免疫治疗的研究。

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED₅₀ for this effect is <1 ng/mL.

Mouse

HEK293

N-His;N-Avi

P04351 (A21-Q169)

16183  [NCBI]

27-30 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.