IL-20R alpha 蛋白, Mouse (HEK293, Fc)

IL-20R alpha Protein (IL20RA) is the alpha subunit of IL-20 receptor, forms functional heterodimers with different subunit protein and involves in STAT3 activation pathway^[1]. The IL20RA/IL20RB dimer is a receptor for IL-19, IL-20 and IL-24, while the IL20RA/IL10RB dimer is a receptor for IL-26^[3]. IL-20R alpha Protein is consists of 553 amino acids (M1-N553) with a transmembrane domain (254-274 a.a.). IL-20R alpha Protein, Mouse (HEK293, Fc) is produced in HEK293 cells with a C-Terminal His-tag.

IL-20R α 又称 IL-20 受体 α 亚基 (IL-20RA)，属于 II 型细胞因子受体家族^[1]。
IL-20R α 广泛表达，在皮肤和睾丸中表达最高，在大脑中表达也较高^[2]。
IL20RA 和 IL20RB 在银屑病皮损的角质形成细胞上表达上调^[3]。
IL-20R α 与不同亚基蛋白形成功能性异二聚体。IL-20Rα通过与IL20RB二聚化而充当IL-19、IL-20和IL-24的受体，然而通过与IL-10RB二聚化而充当IL-26的受体。IL-20R α 至少部分通过 STAT3 激活转导配体结合，并在表皮功能和牛皮癣中发挥重要作用^[4]。
IL-12 中的氨基酸序列小鼠的β蛋白与人类有很大不同（64.64%）。

Overexpression of IL-20 in transgenic (TG) mice causes neonatal lethality with skin abnormalities including aberrant epidermal differentiation^[1].

Mouse IL-20RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-20, His Tag with an affinity constant of 13.93 μM as determined in SPR assay (Biacore T200).

Mouse

HEK293

C-hFc

Q6PHB0 (V33-K253)

237313  [NCBI]

65-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wang F, et al. Prominent production of IL-20 by CD68+/CD11c+ myeloid-derived cells in psoriasis: Gene regulation and cellular effects. J Invest Dermatol. 2006 Jul;126(7):1590-9.  [Content Brief]

  [2]. Blumberg H, et al. Interleukin 20: discovery, receptor identification, and role in epidermal function. Cell. 2001 Jan 12;104(1):9-19.  [Content Brief]

  [3]. Sheikh F, et al. Cutting edge: IL-26 signals through a novel receptor complex composed of IL-20 receptor 1 and IL-10 receptor 2. J Immunol. 2004 Feb 15;172(4):2006-10.  [Content Brief]