IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer 蛋白, Human (Biotinylated, HEK293, His-Avi)

IL-23, collaborating with IL12B, constitutes the pro-inflammatory cytokine IL-23, crucial in innate and adaptive immunity. Released by antigen-presenting cells like dendritic cells or macrophages, IL-23 binds to IL12RB1 and IL23R, activating JAK2 and TYK2. This cascade phosphorylates STAT3 and STAT4, activating pathways like p38 MAPK or NF-kappa-B, inducing pro-inflammatory cytokines. IL-23 contributes to intracellular bacterial clearance and supports the expansion of T-helper 17 cells, including IL-17 producers. The disulfide-linked IL-23 interacts with IL12B and IL23R, recruiting IL12RB1. IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived IL-23 alpha, expressed by HEK293, with C-Avi, C-His labeled tag. IL-23 alpha (170a.a) & IL-12 beta (306a.a) Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of 24 kDa (IL-23 alpha) & 40-50 kDa (IL-12 beta), respectively.

IL-23 与 IL12B 协同形成促炎细胞因子 IL-23，在先天免疫和适应性免疫中发挥多种作用。IL-23 由树突状细胞或巨噬细胞等抗原呈递细胞释放，与包含 IL12RB1 和 IL23R 的异二聚体受体复合物结合，启动涉及 JAK2 和 TYK2 激活的级联反应。这些激酶磷酸化受体，为随后的 STAT3 和 STAT4 磷酸化创建对接位点。该过程激活多种途径，包括 p38 MAPK 或 NF-kappa-B，促进促炎细胞因子的产生，例如 IL-17A/IL17A。此外，IL-23 积极参与细胞内细菌的早期有效清除。值得注意的是，IL-23 促进 T 辅助细胞 17 细胞的扩增和存活，T 辅助细胞 17 是一种以产生 IL-17 的 CD4 阳性辅助 T 细胞亚群，以及其他产生 IL-17 的细胞。IL-23 与 IL12B 的异二聚体（称为白细胞介素 IL-23）是二硫键连接的。此外，IL-23 与 IL23R 相互作用，促进 IL12RB1 的募集。

Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5 μg/mL (100 μl/Well) on streptavidin (5 μg/mL) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC₅₀ of ≤3.6 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

Q9NPF7 (R20-P189)&P29460 (I23-S328)

51561&3593

24 kDa (IL-23 alpha) & 40-50 kDa (IL-12 beta)

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.