IL-25/IL-17E 蛋白, Human (HEK293, His)

IL-25/IL-17E cytokines are produced by eosinophils, Th2 cells, and epithelial cells and critically regulate the adaptive immune response by modulating cytokine production. It enhances local and systemic type 2 helper T cell responses through its IL17RA and IL17RB receptors and activates the JAK2-STAT5A pathway. IL-25/IL-17E Protein, Human (HEK293, His) is the recombinant human-derived IL-25/IL-17E protein, expressed by HEK293 , with C-6*His labeled tag.

细胞因子 Animal-Free IL-25/IL-17E 由嗜酸性粒细胞、辅助性 T 2 型 (Th2) 细胞或上皮细胞等多种细胞产生，通过调节细胞因子，在适应性免疫反应的内部安全性中发挥着至关重要的作用生产。该细胞因子通过由 IL17RA 和 IL17RB 组成的受体发挥信号转导作用，促进并增强局部和全身的 2 型辅助 T 反应。结合后，Animal-Free IL-25 会激活 JAK2-STAT5A 通路，导致 2 型相关细胞因子的分泌，包括 IL4、IL9 和 IL13。此外，它还通过同时激活 MAPK 和 NF-kappa-B 途径诱导嗜酸性粒细胞释放 IL8 和 IL6。值得注意的是，Animal-Free IL-25 通过产生 IL4、IL5 和 IL13 来抑制 T 辅助细胞 (Th17) 的分化，从而有助于其在免疫反应中的调节作用。

Measured by its ability to induce CXCL1 secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 1.231 ng/mL, Corresponding to a specific activity is 8.123×10⁵ U/mg.

Human

HEK293

C-6*His

Q9H293-1 (Y33-G177)

64806  [NCBI]

YSHWPSCCPSKGQDTSEELLRWSTVPVPPLEPARPNRHPESCRASEDGPLNSRAISPWRYELDRDLNRLPQDLYHARCLCPHCVSLQTGSHMDPRGNSELLYHNQTVFYRRPCHGEKGTHKGYCLERRLYRVSLACVCVRPRVMG

20-26 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl,150 mM NaCl 1 mM EDTA, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.