IL-27 蛋白, Mouse (HEK293, C-His)

The IL-27 protein together with IL23A forms IL-23 interleukin, a key cytokine in innate and adaptive immunity. It is associated with infection responses, binds to IL12RB1 and IL23R receptor complexes, and activates Jak-Stat signaling.IL-27 Protein, Mouse (HEK293, C-His) is a recombinant protein dimer complex containing IL-27 Protein and IL-27A, expressed by HEK293 , with C-10*His labeled tag and GSGSGGSGGSGSGKL peptide.

IL-12 与 IL23A 结合形成 IL-23 白细胞介素，这是一种异二聚体细胞因子，在先天性和适应性免疫中发挥重要作用。这种细胞因子参与外周组织感染的急性反应，并与由 IL12RB1 和 IL23R 组成的异二聚体受体复合物结合，激活 Jak-Stat 信号级联。IL-23 刺激记忆 T 细胞而不是幼稚 T 细胞，并促进促炎细胞因子的产生。它已被确定为自身免疫性炎症的一个促成因素，可能导致自身免疫性炎症疾病并与肿瘤发生有关。此外，IL-12 还可作为激活的 T 细胞和 NK 细胞的生长因子，增强 NK/淋巴因子激活的杀伤细胞的裂解活性，并刺激静息 PBMC 产生干扰素-γ。

Measured in a cell proliferation assay using TF-1 Human Erythroleukemia Cells. The ED₅₀ this effect is 52.01 ng/ml, corresponding to a specific activity is 1.923×10^4 units/mg.

• 
  Measured in a cell proliferation assay using TF-1 Human Erythroleukemia Cells. The ED₅₀ for this effect is 52.01 ng/ml, corresponding to a specific activity is 1.923×10⁴ units/mg.

Mouse

HEK293

C-10*His

O35228 (Y19-P228) & GSGSGGSGGSGSGKL & Q8K3I6 (F29-S234)

50498&246779

YTETALVALSQPRVQCHASRYPVAVDCSWTPLQAPNSTRSTSFIATYRLGVATQQQSQPCLQRSPQASRCTIPDVHLFSTVPYMLNVTAVHPGGASSSLLAFVAERIIKPDPPEGVRLRTAGQRLQVLWHPPASWPFPDIFSLKYRLRYRRRGASHFRQVGPIEATTFTLRNSKPHAKYCIQVSAQDLTDYGKPSDWSLPGQVESAPHKPGSGSGGSGGSGSGKLFPTDPLSLQELRREFTVSLYLARKLLSEVQGYVHSFAESRLPGVNLDLLPLGYHLPNVSLTFQAWHHLSDSERLCFLATTLRPFPAMLGGLGTQGTWTSSEREQLWAMRLDLRDLHRHLRFQVLAAGFKCSKEEEDKEEEEEEEEEEKKLPLGALGGPNQVSSQVSWPQLLYTYQLLHSLELVLSRAVRDLLLLSLPRRPGSAWDS

Approximately 57.6 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.