IL-3 蛋白, Mouse (HEK293, His)

IL-3 Protein, Mouse (HEK293, His) is a multilineage hematopoietic cytokine with promising effects on platelet and neutrophil counts and special usefulness in patients with secondary hematopoietic failure.

白细胞介素-3 (IL-3) 是属于造血生长因子家族的糖蛋白，在临床前体外和体内研究中已表现出多谱系活性。重组人白细胞介素 3 (rhIL-3) 通过重组人粒细胞集落刺激因子 (rhG-CSF) 增强外周血祖细胞的动员^[1]。人白细胞介素 3 (hIL-3) 是一种多能造血细胞因子，由有丝分裂原和抗原激活的角质形成细胞、T 淋巴细胞、肥大细胞、NK 细胞、单核细胞和内皮细胞产生。造血祖细胞在hIL-3蛋白的帮助下增殖并分化为成熟红细胞、肥大细胞、巨核细胞和粒细胞。hIL-3 蛋白的潜在用途已在骨髓移植、血液恶性肿瘤、血细胞减少症、再生障碍性贫血和各种类型的癌症等各种临床应用中得到广泛测试^[2]。

Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is <582 pg/mL.

• 
  Measured in a cell proliferation assay using M-NFS-60 cells. The ED₅₀ for this effect is 79.38 pg/mL, corresponding to a specific activity is 1.26×10⁷ units/mg.

Mouse

HEK293

C-6*His

P01586 (A27-C166)

16187  [NCBI]

ASISGRDTHRLTRTLNCSSIVKEIIGKLPEPELKTDDEGPSLRNKSFRRVNLSKFVESQGEVDPEDRYVIKSNLQKLNCCLPTSANDSALPGVFIRDLDDFRKKLRFYMVHLNDLETVLTSRPPQPASGSVSPNRGTVEC

Approximately 25-30 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Huhn RD, et al. Recombinant human interleukin-3 (rhIL-3) enhances the mobilization of peripheral blood progenitor cells by recombinant human granulocyte colony-stimulating factor (rhG-CSF) in normal volunteers. Exp Hematol. 1996 Jun;24(7):839-47.  [Content Brief]

  [2]. Dagar VK, et al. Combined effect of gene dosage and process optimization strategies on high-level production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris fed-batch culture. Int J Biol Macromol. 2018 Mar;108:999-1009.  [Content Brief]