IL-3 蛋白, Mouse (HEK293)

IL-3 protein is secreted by T lymphocytes, mast cells and osteoblasts. It plays an important regulatory role in hematopoietic progenitor cells and stimulates mature basophils, eosinophils and monocytes. In addition to hematopoiesis, it supports neuronal cell proliferation, survival, and bone homeostasis by inhibiting osteoclast differentiation. IL-3 Protein, Mouse (HEK293) is the recombinant mouse-derived IL-3 protein, expressed by HEK293 , with tag free.

细胞因子 IL-3 主要由活化的 T 淋巴细胞、肥大细胞和成骨细胞分泌，在控制造血祖细胞向谱系限制细胞的产生和分化中发挥着至关重要的作用。此外，IL-3 可刺激成熟的嗜碱性粒细胞、嗜酸性粒细胞和单核细胞，促进其功能激活。除造血功能外，IL-3 还有助于神经细胞增殖和存活，并通过防止 NF-kappa-B 核易位和激活来抑制破骨细胞分化，从而参与骨稳态。从机制上讲，IL-3 通过由 IL3RA 亚基和信号转导亚基 IL3RB 组成的受体发挥其生物效应，导致 JAK2 激酶活性快速激活以及随后 STAT5 介导的转录编程。此外，IL-3 作为单体，通过激活 PI3K/AKT 和 ERK 介导的途径，有助于非造血系统中氧化应激下的细胞存活。

Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells and the ED₅₀ is 0.1-0.5 ng/mL.

Mouse

HEK293

Tag Free

P01586 (A27-C166)/NP_034686.2

16187  [NCBI]

MVLASSTTSIHTMLLLLLMLFHLGLQASISGRDTHRLTRTLNCSSIVKEIIGKLPEPELKTDDEGPSLRNKSFRRVNLSKFVESQGEVDPEDRYVIKSNLQKLNCCLPTSANDSALPGVFIRDLDDFRKKLRFYMVHLNDLETVLTSRPPQPASGSVSPNRGTVEC

Approximately 15.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.