IL-4R alpha/CD124 蛋白, Rat (HEK293, Fc)

IL-4R alpha is a subunit alpha shared by IL-4 and IL-13 receptors, found in leukocytes originally. IL-4R alpha couples to the JAK1/2/3-STAT6 pathway and involves in promoting Th2 differentiation^[1]. IL-4R alpha/CD124, Rat consists of 801 amino acids (M1-S801), exerts IL-4 binding function and results the phosphorylation of C-terminal tyrosine residues. IL-4R alpha/CD124, Rat has an immunoreceptor tyrosine inhibitor motif (ITIM) (698-703 a.a), which can bind to the SH2 domain of some phosphatases by phosphorylating ITIM. IL-4R alpha/CD124, Rat has a a soluble form (1-229 a.a) and a transmembrane domain (233-256 a.a). The soluble IL-4R (sIL4R) inhibits IL4-induced spleen cell proliferation. IL-4R alpha/CD124, Rat is produced in HEK293 cells, including a Fibronectin typr III domain (125-223 a.a) and a C-Terminal hFc-tag.

白介素 4R α (IL-4Rα) 也称为 CD124 和 B 细胞刺激因子 (BSF) 受体，是一种抗炎细胞因子，在活化的 T 细胞中高表达^[1]。
IL-4R α 参与不同细胞类型中两种白细胞介素受体的形成。对于I型受体，依赖于IL-4Rα结合IL-4来招募免疫细胞中的IL-2Rγ链。IL-2R γ 是多种白细胞介素受体的常见亚基，参与 IL-15 刺激中性粒细胞的吞噬作用。对于II型受体，依赖IL-4Rα结合IL-4来募集IL-13Rα1链。IL-13R α 1 是非免疫细胞中常见细胞因子受体 γ 链的替代辅助蛋白^[2][3]。
大鼠 IL-4R α 蛋白中的氨基酸与人类 (52.82%) 和小鼠 (79.53%) 的同源性较低。
IL-4 R α 通过交替剪接或蛋白水解产生可溶形式，保持配体结合特性并抑制IL-4的生物活性。IL-4 R α 可溶性亚型 1 可以通过金属蛋白酶在细胞表面进行蛋白水解裂解（脱落）来产生^[4]。
IL-4 R α 在 Th2 中发挥重要作用- 免疫反应偏向、巨噬细胞活化、粘膜免疫、过敏性炎症、肿瘤进展和动脉粥样硬化^[5]。

IL-4 R alpha shows a high R/S value in extracellular domain that indicates selective pressure for amino acid exchanges, and shows a low R/S value for the intracellular part^[1].
IL-4 R alpha can be cleaved into a soluble interleukin-4 receptor, as sIL-4R, is expressed in kidney cells and antagonistically functional on IL-4-induced proliferation of spleen cells^[2].
sIL-4R is found not only in rats spleen cells but also in their glomerular epithelial cells (GEC)^[2].

IL-4 R alpha shows a high R/S value in extracellular domain that indicates selective pressure for amino acid exchanges, and shows a low R/S value for the intracellular part^[6].
IL-4 R alpha can be cleaved into a soluble interleukin-4 receptor, as sIL-4R, is expressed in kidney cells and antagonistically functional on IL-4-induced proliferation of spleen cells^[7].
sIL-4R is found not only in rats spleen cells but also in their glomerular epithelial cells (GEC)^[7].

Measured by its binding ability in a functional ELISA. Immobilized mouse IL4-His at 10 μg/mL (100 μL/well) can bind rat IL4R-Fc and the EC₅₀ is 0.12-0.29 μg/mL.

Rat

HEK293

C-hFc

Q63257-1 (I26-R232)

25084  [NCBI]

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Keegan AD,et al. An IL-4 receptor region containing an insulin receptor motif is important for IL-4-mediated IRS-1 phosphorylation and cell growth. Cell. 1994 Mar 11;76(5):811-20.  [Content Brief]

  [2]. Zurawski SM, et al. The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor. J Biol Chem. 1995 Jun 9;270(23):13869-78.  [Content Brief]

  [3]. Rolling C, et al. IL4 and IL13 receptors share the gamma c chain and activate STAT6, STAT3 and STAT5 proteins in normal human B cells. FEBS Lett. 1996 Sep 9;393(1):53-6.  [Content Brief]

  [4]. Jung T, et al. Soluble human interleukin-4 receptor is produced by activated T cells under the control of metalloproteinases. Int Arch Allergy Immunol. 1999 May;119(1):23-30.  [Content Brief]

  [5]. HageT,etal.Crystalstructureoftheinterleukin-4/receptoralphachaincomplexrevealsamosaicbindinginterface.Cell.1999Apr16;97(2):271-81.  [Content Brief]

  [6]. Richter G, et al. The rat interleukin 4 receptor: coevolution of ligand and receptor. Cytokine. 1995 Apr;7(3):237-41.  [Content Brief]

  [7]. Guangping, et al. Identification of soluble interleukin-4 receptor in rat glomerular epithelial cells[J]. Biochimica Et Biophysica Acta Molecular Cell Research, 1999.