IL-5 蛋白, Human

IL-5 Protein, Human is a selective activator of human eosinophil function.

重组人白细胞介素 5 (rhIL-5) 是一种强效、选择性的人嗜酸性粒细胞功能刺激剂。因此，IL-5 是第一个造血因子，其在体内的研究可以解释疾病中所见的选择性嗜酸性粒细胞增多和嗜酸性粒细胞活化^[1]。重组人白细胞介素-5 (rhIL-5)，在液体或半固体培养物中，选择性诱导正常人骨髓产生嗜酸性粒细胞，而对其他细胞谱系没有活性。人白细胞介素 5 (IL-5) 调节人骨髓培养物中嗜酸性粒细胞的产生：与 IL-1、IL-3、IL-6 和粒细胞巨噬细胞集落刺激因子 (GMCSF) 的比较和相互作用^[2]。

The ED₅₀ is <1.0 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >1.0 × 10⁶ units/mg.

Human

E. coli

Tag Free

P05113 (I20-S134)

3567  [NCBI]

MIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES

Approximately 26.5 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 25 mM Tris, pH 8.0.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Lopez AF, et al. Recombinant human interleukin 5 is a selective activator of human eosinophil function. J Exp Med. 1988 Jan 1;167(1):219-24.  [Content Brief]

  [2]. Clutterbuck EJ, et al. Human interleukin-5 (IL-5) regulates the production of eosinophils in human bone marrowcultures: comparison and interaction with IL-1, IL-3, IL-6, and GMCSF. Blood. 1989 May 1;73(6):1504-12.  [Content Brief]