IL-5 蛋白, Rat (CHO)

IL-5 Protein, Rat (CHO), a CHO cell derived cytokine, stimulates B cell growth and increases immunoglobulin secretion.

白介素 5 (IL-5) 是一种 T 细胞衍生的细胞因子，可刺激人类、小鼠和绵羊的嗜酸性粒细胞产生和活化。IL-5 作为小鼠 B 细胞的生长因子具有活性，但对人类 B 细胞没有活性。IL-5 对反刍动物 B 细胞的作用尚未明确定义。通过与人 IL-5 cDNA 杂交，从肝脏基因组文库中分离出绵羊 IL-5 基因。IL-5 cDNA 通过逆转录酶 PCR 获得，使用根据源自绵羊 IL-5 基因的 5' 和 3' 编码序列设计的引物。cDNA序列显示与人和小鼠序列有79%和73%的核苷酸同源性。绵羊IL-5 cDNA编码132个氨基酸的蛋白质，与人和小鼠IL-5的氨基酸同源性水平分别为64％和56％。绵羊、人和小鼠 IL-5 中有两个半胱氨酸残基是保守的。绵羊 IL-5^[1] 中有两个潜在的 N 联糖基化位点。

The ED₅₀ is <0.5 ng/mL as measured by TF-1 cells.

Rat

CHO

Tag Free

Q08125 (M20-V132)

24497  [NCBI]

MEIPMSTVVKETLIQLSTHRALLTSNETMRLPVPTHKNHQLCIGEIFQGLDILKNQTVRGGTVEILFQNLSLIKKYIDGQKEKCGEERRKTRHFLDYLQEFLGVMSTEWAMEV

13-21 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Seow HF, et al. Cloning and sequencing of an ovine interleukin-5 cDNA. DNA Seq. 1996;6(6):331-5.  [Content Brief]