IL-7 蛋白, Mouse (HEK293, His)

IL-7, a hematopoietic cytokine, regulates lymphocyte population and maintains lymphoid homeostasis. IL-7 binds to its receptor complex IL7RA and CSF2RG, activating kinases like JAK1 or JAK3 depending on the cell type. This triggers downstream signaling pathways, including PI3K/Akt/mTOR or JAK-STAT5, leading to various cellular responses. IL-7's interaction with IL7R and CSF2RG is crucial for its diverse effects. IL-7 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-7 protein, expressed by HEK293 , with C-6*His labeled tag.

IL-7 是一种造血细胞因子，在幼稚 T 细胞和记忆 T 细胞和 B 细胞的发育、扩增和存活中发挥着关键作用，从而调节成熟淋巴细胞的数量并维持淋巴稳态。其生物学效应是通过由 IL7RA 亚基和细胞因子受体共同亚基 gamma/CSF2RG 组成的受体复合物介导的。与受体结合后，IL-7 会根据细胞类型激活各种激酶，例如 JAK1 或 JAK3，从而导致信号通过下游信号通路（包括 PI3K/Akt/mTOR 或 JAK-STAT5）传播。这种与其受体成分 IL7R 和 CSF2RG 的相互作用是 IL-7 引发的多种细胞反应的核心。

1.Mouse IL-7RA-Fc can bind Mouse IL-7-His with an affinity constant of 18.9 nM.
2.Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL).The ED₅₀ for this effect is 60-1000 pg/mL.

Mouse

HEK293

C-6*His

P10168/Q544C8/NP_032397.1 (E26-I154)

16196  [NCBI]

ECHIKDKEGKAYESVLMISIDELDKMTGTDSNCPNNEPNFFRKHVCDDTKEAAFLNRAARKLKQFLKMNISEEFNVHLLTVSQGTQTLVNCTSKEEKNVKEQKKNDACFLKRLLREIKTCWNKILKGSI

22-28 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.