ILKAP/PP2C delta 蛋白, Human (HEK293, His)

The ILKAP/PP2C delta protein is a key phosphatase that potentially regulates cell cycle progression by dephosphorylating substrates essential for proliferation. It selectively binds to ILK and regulates cell adhesion and growth factor signaling. ILKAP/PP2C delta Protein, Human (HEK293, His) is the recombinant human-derived ILKAP/PP2C delta protein, expressed by HEK293 , with C-His labeled tag. The total length of ILKAP/PP2C delta Protein, Human (HEK293, His) is 392 a.a..

ILKAP/PP2C δ 蛋白作为一种关键磷酸酶出现，通过对细胞增殖至关重要的底物进行去磷酸化，对调节细胞周期进程具有潜在影响。这种蛋白质选择性地与整合素连接激酶 (ILK) 结合，对细胞粘附和生长因子信号传导发挥调节作用。ILKAP/PP2C delta 特异性抑制 ILK-GSK3B 信号轴，表明其在抑制该通路的活性中发挥作用。这种抑制可能在阻止致癌转化中发挥关键作用，强调了 ILKAP/PP2C delta 作为潜在肿瘤抑制因子的重要性。ILKAP/PP2C delta、ILK 和下游信号通路之间复杂的相互作用强调了其在基本细胞过程中的调节作用，对细胞周期动力学和致癌转化控制都有影响。

Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO3)VAAE. The specific activity is 562.344 nmol/min/mg.

Human

HEK293

C-6*His

Q9H0C8 (M1-H392)

80895  [NCBI]

MDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHVILNDITEECRPPSSLITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQKPAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRDGRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKIQTREGKSAADARYEAACNRLANKAVQRGSADNVTVMVVRIGH

Approximately 46 kDa.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.