2. 重组蛋白
  3. CD Antigens Receptor Proteins
  4. NK Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Leukocyte Immunoglobin-like Receptors
  5. CD85e/LILRA3 CD85e/LILRA3
  6. ILT6 蛋白, Human (HEK293, His)

ILT6 蛋白, Human (HEK293, His)

目录号: HY-P70162
产品使用指南

LILRA3/CD85e/ILT6 作为 I 类 MHC 抗原的可溶性受体发挥作用，结合经典和非经典 HLA I 类分子，尽管亲和力低于 LILRB1 或 LILRB2。它与单核细胞表面结合，有效抑制单核细胞 LPS 诱导的 TNF-α 产生。ILT6 蛋白, Human (HEK293, His) 是重组的 ILT6 蛋白，由 HEK293 表达，带有 C-6*His 标签。

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描述

LILRA3/CD85e/ILT6 functions as a soluble receptor for class I MHC antigens, binding both classical and non-classical HLA class I molecules, albeit with lower affinities than LILRB1 or LILRB2. It engages with monocyte surfaces, effectively suppressing LPS-induced TNF-alpha production by monocytes. ILT6 Protein, Human (HEK293, His) is the recombinant human-derived ILT6 protein, expressed by HEK293 , with C-6*His labeled tag.
研究背景

LILRA3/CD85e/ILT6 作为 I 类 MHC 抗原的可溶性受体，结合经典和非经典 HLA I 类分子，但与 LILRB1 或 LILRB2 相比亲和力降低。该蛋白与单核细胞表面表现出高亲和力相互作用，从而抑制单核细胞 LPS 诱导的 TNF-α 产生。
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

Q8N6C8-1/AAH28208.1 (T19-E439)
基因 ID
蛋白结构
N-term
ILT6 (T19-E439)
Accession # Q8N6C8-1/AAH28208.1
6*His
C-term
同用名
rHuLeukocyte immunoglobulin-like receptor subfamily A member 3/ILT6, His; Leukocyte immunoglobulin-like receptor subfamily A member 3; CD85 antigen-like family member E; Immunoglobulin-like transcript 6; ILT-6; Leukocyte immunoglobulin-like receptor 4; LIR-4 and Monocyte inhibitory receptor HM43/HM31
氨基酸序列

THVQAGPLPKPTLWAEPGSVITQGSPVTLRCQGSLETQEYHLYREKKTALWITRIPQELVKKGQFPILSITWEHAGRYCCIYGSHTAGLSESSDPLELVVTGAYSKPTLSALPSPVVTSGGNVTIQCDSQVAFDGFILCKEGEDEHPQCLNSHSHARGSSRAIFSVGPVSPSRRWSYRCYGYDSRAPYVWSLPSDLLGLLVPGVSKKPSLSVQPGPVVAPGEKLTFQCGSDAGYDRFVLYKEWGRDFLQRPGRQPQAGLSQANFTLGPVSRSYGGQYTCSGAYNLSSEWSAPSDPLDILITGQIRARPFLSVRPGPTVASGENVTLLCQSQGGMHTFLLTKEGAADSPLRLKSKRQSHKYQAEFPMSPVTSAHAGTYRCYGSLSSNPYLLTHPSDPLELVVSGAAETLSPPQNKSDSKAGE

分子量

Approximately 70.0 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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