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  3. Immunoglobulin J chain 蛋白, Mouse (P.pastoris, His)

Immunoglobulin J chain 蛋白, Mouse (P.pastoris, His)

目录号: HY-P71744
产品使用指南

免疫球蛋白 J 链连接 IgMIgA 单体。在 IgM 中,J 链连接的二聚体使 IgM 五聚体成核,而在 IgA 中,它诱导二聚体或多聚体形成。它还将这些免疫球蛋白与分泌成分结合。作为 sIgA 复合物的一部分,它与 IgA 单体、J 链和分泌成分(PIGR 蛋白水解产物)结合。Immunoglobulin J chain 蛋白, Mouse (P.pastoris, His) 是重组的 免疫球蛋白 J 链蛋白,由 P. pastoris 表达,带有 N-His 标签。Immunoglobulin J chain 蛋白, Mouse (P.pastoris, His) 全长 138 个氨基酸,分子量约为 ~17.7 kDa。

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描述

The Immunoglobulin J chain links IgM or IgA monomers. In IgM, J chain-joined dimers nucleate the IgM pentamer, while in IgA, it induces dimer or polymer formation. It also binds these immunoglobulins to the secretory component. As part of the sIgA complex, it integrates with IgA monomers, the J chain, and the secretory component (PIGR proteolytic product). Immunoglobulin J chain Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Immunoglobulin J chain protein, expressed by P. pastoris , with N-His labeled tag. The total length of Immunoglobulin J chain Protein, Mouse (P.pastoris, His) is 138 a.a., with molecular weight of ~17.7 kDa.

研究背景

免疫球蛋白 J 链是连接 IgMIgA 两个单体单元的关键环节。在 IgM 的情况下,J 链连接的二聚体充当 IgM 五聚体的成核单元,而对于 IgA,它诱导二聚体和/或更大的聚合物的形成。此外,免疫球蛋白 J 链在将这些免疫球蛋白与分泌成分结合方面发挥着作用。它是分泌型 IgA (sIgA) 复合物的组成部分,该复合物包含两个、四个或五个 IgA 单体和两个额外的非 Ig 多肽,即 J 链和分泌成分(PIGR 的蛋白水解产物)。

种属

Mouse

表达系统

P. pastoris

标签

N-His

蛋白编号

P01592 (22G-159D)

基因 ID
蛋白结构
N-term
His
J-chain (22G-159D)
Accession # P01592
C-term
同用名
Jchain; IgjImmunoglobulin J chain
氨基酸序列

GDDEATILADNKCMCTRVTSRIIPSTEDPNEDIVERNIRIVVPLNNRENISDPTSPLRRNFVYHLSDVCKKCDPVEVELEDQVVTATQSNICNEDDGVPETCYMYDRNKCYTTMVPLRYHGETKMVQAALTPDSCYPD

分子量

Approximately 17.7 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Immunoglobulin J chain Protein, Mouse (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Immunoglobulin J chain Protein, Mouse (P.pastoris, His)
目录号:
HY-P71744
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