Integrin alpha 5 beta 1 蛋白, Human (HEK293, His-Avi)

ITGA5 is a multifunctional receptor that interacts with multiple ligands such as fibronectin, fibrinogen, PLA2G2A, fibrillin-1 (FBN1), IL1B, human metapneumovirus, and soluble CD40LG. ITGA5 recognizes RGD sequences and mediates cell adhesion through different binding sites. Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived Integrin alpha 5 beta 1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi), has molecular weight of 100-140 kDa.

整合素 α-5/β-1 蛋白 (ITGA5:ITGB1) 作为具有多种配体相互作用的多功能受体。ITGA5:ITGB1 识别其配体中的 RGD 序列，充当纤连蛋白和纤维蛋白原的受体，通过不同的结合位点介导细胞粘附。值得注意的是，它在与其经典配体结合位点不同的位点上与 PLA2G2A 结合，从而诱导增强配体结合的构象变化。此外，ITGA5:ITGB1 作为 fibrillin-1 (FBN1) 的受体，促进 RGD 依赖性细胞粘附。它也是纤连蛋白 (FN1) 的受体，使 RGD 依赖性细胞粘附到 FN1。此外，ITGA5:ITGB1 作为 IL1B 受体，在 IL1B 信号传导中发挥着至关重要的作用。在微生物感染的情况下，ITGA5:ITGB1 作为人类偏肺病毒的受体，强调了它参与病原体识别。此外，ITGA5:ITGB3 作为可溶性 CD40LG 的受体，在 CD40/CD40LG 信号传导中发挥着至关重要的作用。这种广泛的配体相互作用强调了 ITGA5:ITGB1 在细胞过程和信号通路中的多功能性。

Immobilized Human ITGA5&ITGB1, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC₅₀ of <25.8 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-8*His

P08648 (F42-Y995)&P05556 (Q21-D728)

3678  [NCBI]&3688  [NCBI]

100-140 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.