Integrin alpha 8 beta 1 蛋白, Human (HEK293, Flag, His)

Integrin alpha-8/beta-1 orchestrates mesenchymal cell recruitment during kidney organogenesis, recognizing the R-G-D sequence in ligands like TNC, FN1, SPP1, TGFB1, TGFB3, and VTN. Its role in diverse cellular interactions, crucial for organ formation, includes NPNT as a functional ligand in kidney genesis. As a neuronal receptor for TNC, it modulates neurite outgrowth in sensory and motor neurons. Structurally, Integrin alpha-8/beta-1 forms a heterodimer with the alpha-8 subunit associating with the beta-1 subunit, emphasizing its integral role in cell-matrix and cell-cell interactions during organ development and neural processes. Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His) is a recombinant protein dimer complex containing human-derived Integrin alpha 8 beta 1 protein, expressed by HEK293, with C-10*His, C-Flag labeled tag. Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His), has molecular weight of ~85-140 kDa.

整合素 α-8/β-1 蛋白通过协调间充质细胞招募到上皮结构中，在器官发生中发挥着至关重要的作用，特别是在肾脏和其他潜在器官的发育中。整合素 α-8/β-1 识别多种配体（包括 TNC、FN1、SPP1、TGFB1、TGFB3 和 VTN）中的 RGD 序列，与对器官形成至关重要的多种细胞相互作用有关。在肾脏发生过程中，NPNT 被认为是其可能的功能配体。作为 TNC 的神经元受体，它调节细胞间相互作用并调节感觉和运动神经元的神经突生长。在结构上，整合素α-8/β-1形成异二聚体，其中α亚基包含通过二硫键连接的重链和轻链。具体来说，α-8 亚基与 β-1 亚基相关，强调了其在器官发育和神经过程中介导细胞-基质和细胞-细胞相互作用中的不可或缺的作用。

Measured by its binding ability in a functional ELISA. Immobilized Human Fibronectin, at 5 μg/mL (100 μL/well) can bind Biotinylated Human Integrin alpha 8 beta 1 protein. The ED₅₀ for this effect is 428.7 ng/mL

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  Measured by its binding ability in a functional ELISA. Immobilized Human Fibronectin, at 5 μg/mL (100 μL/well) can bind Biotinylated Human Integrin alpha 8 beta 1 protein. The ED₅₀ for this effect is 428.7 ng/mL .

Human

HEK293

C-10*His;C-Flag

P53708 (F39-L1012)&P05556-1 (Q21-D728)

8516  [NCBI]& 3688  [NCBI]

FNLDVEKLTVYSGPKGSYFGYAVDFHIPDARTASVLVGAPKANTSQPDIVEGGAVYYCPWPAEGSAQCRQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGATVKAHKGKVVACAPLYHWRTLKPTPEKDPVGTCYVAIQNFSAYAEFSPCRNSNADPEGQGYCQAGFSLDFYKNGDLIVGGPGSFYWQGQVITASVADIIANYSFKDILRKLAGEKQTEVAPASYDDSYLGYSVAAGEFTGDSQQELVAGIPRGAQNFGYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNSDGLDDVLVGAPLFMEREFESNPREVGQIYLYLQVSSLLFRDPQILTGTETFGRFGSAMAHLGDLNQDGYNDIAIGVPFAGKDQRGKVLIYNGNKDGLNTKPSQVLQGVWASHAVPSGFGFTLRGDSDIDKNDYPDLIVGAFGTGKVAVYRARPVVTVDAQLLLHPMIINLENKTCQVPDSMTSAACFSLRVCASVTGQSIANTIVLMAEVQLDSLKQKGAIKRTLFLDNHQAHRVFPLVIKRQKSHQCQDFIVYLRDETEFRDKLSPINISLNYSLDESTFKEGLEVKPILNYYRENIVSEQAHILVDCGEDNLCVPDLKLSARPDKHQVIIGDENHLMLIINARNEGEGAYEAELFVMIPEEADYVGIERNNKGFRPLSCEYKMENVTRMVVCDLGNPMVSGTNYSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNPDSNFVSLQINITAVAQVEIRGVSHPPQIVLPIHNWEPEEEPHKEEEVGPLVEHIYELHNIGPSTISDTILEVGWPFSARDEFLLYIFHIQTLGPLQCQPNPNINPQDIKPAASPEDTPELSAFLRNSTIPHLVRKRDVHVVEFHRQSPAKILNCTNIECLQISCAVGRLEGGESAVLKVRSRLWAHTFLQRKNDPYALASLVSFEVKKMPYTDQPAKLPEGSIVIKTSVIWATPNVSFSIPL&QTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPD

Approximately 85-140 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.