Intrinsic Factor/GIF 蛋白, Human (HEK293, His)

Intrinsic factor (GIF) plays a crucial role in cobalamin (Cbl) absorption in the ileum. This well-coordinated process involves the interaction of the CBLIF-cobalamin complex with the Cubilin (CUBN) receptor, leading to internalization via receptor-mediated endocytosis. Intrinsic Factor/GIF Protein, Human (HEK293, His) is the recombinant human-derived Intrinsic Factor/GIF protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of Intrinsic Factor/GIF Protein, Human (HEK293, His) is 399 a.a., with molecular weight of ~49 kDa.

内在因子 (GIF) 是回肠吸收必需维生素钴胺素 (Cbl) 的关键促进剂。其关键作用通过精心策划的过程展开，其中 CBLIF-钴胺素复合物在与 Cubilin (CUBN) 受体相互作用后，通过受体介导的内吞作用进行内化。这种错综复杂的相互作用强调了 GIF 在确保钴胺素有效吸收方面的重要性，钴胺素是各种生理过程的重要维生素。GIF 与 CUBN 的相互作用，特别是涉及 CUB 结构域，凸显了控制维生素吸收这一重要方面的分子复杂性。

Human

HEK293

C-6*His

P27352-1 (S19-Y417)

2694  [NCBI]

STQTQSSCSVPSAQEPLVNGIQVLMENSVTSSAYPNPSILIAMNLAGAYNLKAQKLLTYQLMSSDNNDLTIGQLGLTIMALTSSCRDPGDKVSILQRQMENWAPSSPNAEASAFYGPSLAILALCQKNSEATLPIAVRFAKTLLANSSPFNVDTGAMATLALTCMYNKIPVGSEEGYRSLFGQVLKDIVEKISMKIKDNGIIGDIYSTGLAMQALSVTPEPSKKEWNCKKTTDMILNEIKQGKFHNPMSIAQILPSLKGKTYLDVPQVTCSPDHEVQPTLPSNPGPGPTSASNITVIYTINNQLRGVELLFNETINVSVKSGSVLLVVLEEAQRKNPMFKFETTMTSWGLVVSSINNIAENVNHKTYWQFLSGVTPLNEGVADYIPFNHEHITANFTQY

Approximately 49 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.