IP-10/CRG-2/CXCL10 蛋白, Mouse

CXCL10, also known as interferon γ-induced protein 10 kDa (IP-10), is a cytokine belonging to the CXC chemokine family. CXCL10 exerts its biological effects by binding to CXCR3. CXCL10 is a pleiotropic molecule capable of exerting potent biological functions, including promoting the chemotactic activity of CXCR3+ cells, inducing apoptosis, regulating cell growth and proliferation as well as angiogenesis in infectious and inflammatory diseases and cancer^[1]. IP-10/CRG-2/CXCL10 Protein, Mouse consists of 77 amino acids (I22-P98) and is expressed in E. coli.

CXCL10 是一种由多种细胞分泌的促炎趋化因子。CXCL10 激活 T 淋巴细胞 (Th1)、NK 细胞、巨噬细胞、树突状细胞和 B 细胞。CXCL10 表达水平的变化与炎症性疾病有关，包括传染病、血管生成、免疫功能障碍和肿瘤发展^[1]。
成熟的人类 CXCL10 与小鼠和小鼠有 68% 的氨基酸序列同一性。大鼠 CXCL10。
人类 CXCL10 基因最初由 Lustre 于 1985 年用重组 IFN-γ 处理淋巴瘤细胞系 (U937) 时分离得到。CXCL10 cDNA具有1173bp的开放阅读框，包含4个外显子，编码98个氨基酸的蛋白质。CXCL10 基因的主要翻译产物是含有两个内部二硫键跨桥的 12 kDa 蛋白质。CXCL10 通过与 CXCR3 结合来发挥其生物学作用，CXCR3 是一种以旁分泌或自分泌方式的七跨膜 G 蛋白偶联受体，主要表达于活化的 T、B 淋巴细胞、自然杀伤 (NK)、树突状细胞和巨噬细胞上。CXCL10 诱导主要取决于 CXCR3 的羧基末端区域，这对于 CXCL10 配体诱导的 CXCR3 内化、趋化性和钙动员至关重要。CXCL10 对活化淋巴细胞的强大趋化作用使其能够调节先天性和适应性免疫，诱导组织损伤并调节肿瘤形成^[1]。
CXCL10 是一种多效性分子，能够发挥有效的作用生物学功能，包括促进CXCR3+细胞的趋化活性、诱导细胞凋亡、调节细胞生长和增殖以及感染性和炎症性疾病以及癌症中的血管生成。ELR 阴性 CXCL10 是一种血管抑制趋化因子，可抑制血管生成。在感染病毒、细菌、真菌和寄生虫的个体体液中观察到 CXCL10 水平异常^[1]。

Recombinant mouse CXCL10 protein (1, 10, or 100 ng/mL; for 72 h) shows strongly chemotactic to the astrocytes^[4].

Mice are pretreated with recombinant mouse CXCL10 (2 mg/kg/day, i.v.) or vehicle for 3 days, and injected with BrdU 24 h after final pretreatment. CXCL10 inhibits crypt cell proliferation and migration in vivo^[2].
In Bleomycin-treated mice, recombinant mouse CXCL10 (1 μg/20 μL; daily; for 13 days) is given to mice intramuscularly daily. CXCL10 inhibits primary lung fibroblast migration during fibrosis in WT mice, but not in Sdc4^–/– mice^[3].

1.Full biological activity determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 0.1-10.0 ng/ml in the presence of IL-2.
2.Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED₅₀ for this effect is 0.2587 µg/mL.

Mouse

E. coli

Tag Free

P17515 (I22-P98)

15945  [NCBI]

IPLARTVRCNCIHIDDGPVRMRAIGKLEIIPASLSCPRVEIIATMKKNDEQRCLNPESKTIKNLMKAFSQKRSKRAP

Approximately 8.7-11 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 2× PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Liu M, et al. CXCL10/IP-10 in infectious diseases pathogenesis and potential therapeutic implications. Cytokine Growth Factor Rev. 2011 Jun;22(3):121-30.  [Content Brief]

  [2]. Liu M, et al. CXCL10/IP-10 in infectious diseases pathogenesis and potential therapeutic implications. Cytokine Growth Factor Rev. 2011 Jun;22(3):121-30.  [Content Brief]

  [3]. Shunya Sasaki, et al. Blockade of CXCL10 protects mice from acute colitis and enhances crypt cell survival. Eur J Immunol. 2002 Nov;32(11):3197-205.  [Content Brief]

  [4]. Dianhua Jiang, et al. Inhibition of pulmonary fibrosis in mice by CXCL10 requires glycosaminoglycan binding and syndecan-4. J Clin Invest. 2010 Jun;120(6):2049-57.  [Content Brief]

  [5]. Shinrye Lee, et al. Lipocalin-2 Is a chemokine inducer in the central nervous system: role of chemokine ligand 10 (CXCL10) in lipocalin-2-induced cell migration. J Biol Chem. 2011 Dec 23;286(51):43855-43870.  [Content Brief]