1. 重组蛋白
  2. Enzymes & Regulators
  3. Protein Tyrosine Kinases
  4. IL-2 Inducible T-Cell Kinase (ITK/TSK)
  5. ITK 蛋白, Mouse (sf9, His-GST)

ITK 蛋白, Mouse (sf9, His-GST)

目录号: HY-P74795
COA 产品使用指南

ITK 蛋白是一种酪氨酸激酶,通过控制常规 T 细胞和非常规 NKT 细胞的发育、功能和分化,在指导适应性免疫反应中发挥着至关重要的作用。T细胞受体(TCR)被抗原呈递细胞(APC)激活后,ITK被募集至细胞膜并被LCK磷酸化,从而使其完全激活。ITK 蛋白, Mouse (sf9, His-GST) 是重组的 ITK 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。

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规格 价格 是否有货 数量
50 μg ¥3500 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

ITK protein is a tyrosine kinase that plays a crucial role in directing adaptive immune responses by controlling the development, function, and differentiation of conventional T cells and unconventional NKT cells.After T cell receptor (TCR) activation by antigen-presenting cells (APC), ITK is recruited to the cell membrane and phosphorylated by LCK, thereby making it fully activated.ITK Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived ITK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

研究背景

ITK 蛋白是一种酪氨酸激酶,通过控制传统 T 细胞和非常规 NKT 细胞的发育、功能和分化,在协调适应性免疫反应中发挥着关键作用。抗原呈递细胞 (APC) 激活 T 细胞受体 (TCR) 后,ITK 被募集到细胞膜上,靠近受刺激的 TCR 受体,并在其中被 LCK 磷酸化。这种磷酸化会引发 ITK 自磷酸化,最终导致其完全激活。一旦被激活,ITK 就会磷酸化 PLCG1,从而激活该脂肪酶并触发其底物的后续裂解。因此,钙从内质网释放到细胞质中,促进活化 T 细胞核激活剂 (NFAT) 易位到细胞核中以执行其转录功能。ITK 还会磷酸化两种必需的衔接蛋白,即用于激活 T 细胞/LAT 蛋白和 LCP2 的连接蛋白,从而导致各种信号分子(例如 VAV1)的募集。这种复杂的信号级联最终导致淋巴因子的产生、T 细胞增殖和分化。此外,ITK对于γ-δ T细胞中TCR介导的钙反应是不可或缺的,可能影响未成熟γ-δ T细胞的Vgamma2阳性亚群中的转录组特征。此外,ITK 在“Tyr-525”处磷酸化 TBX21,介导其与 GATA3 的相互作用。ITK 的多方面功能强调了其在塑造适应性免疫反应中的关键作用。

生物活性

The specific activity of ITK was determined to be 54.33 nmol/min/mg using a myelin basic protein (MBP) substrate.

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

Q03526 (R351-L619)

基因 ID
蛋白结构
N-term
His-GST
ITK (R351-L619)
Accession # Q03526
C-term
同用名
Tyrosine-protein kinase ITK/TSK; IL-2-inducible T-cell kinase; ITK; EMT; LYK
分子量

Approximately 58 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.5, 10% Glycerol, 0.5 mM GSH. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ITK Protein, Mouse (sf9, His-GST)
目录号:
HY-P74795
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