Kallikrein-11 蛋白, Mouse (HEK293, His)

Kallikrein-11 protein, a potential multifunctional protease, demonstrates efficient cleavage of the substrate 'bz-Phe-Arg-4-methylcoumaryl-7-amide,' specific to kallikrein. It also displays weaker cleavage activity toward other substrates linked to kallikrein and trypsin. This multifunctional characteristic indicates versatility in substrate recognition and cleavage, suggesting a potential role in diverse proteolytic processes. Kallikrein-11 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kallikrein-11 protein, expressed by HEK293 , with C-His labeled tag.

Kallikrein-11 蛋白被认为是一种潜在的多功能蛋白酶，具有裂解“bz-Phe-Arg-4-methylcoumaryl-7-amide”（一种激肽释放酶特异性底物）的功效。此外，它对与激肽释放酶和胰蛋白酶相关的其他底物表现出较弱的裂解活性。这种多功能性质表明底物识别和切割具有多功能性，突出了其在各种蛋白水解过程中的潜在作用。

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is 335.177 pmol/min/μg, as measured under the described conditions.

Mouse

HEK293

C-6*His

Q9QYN3-1 (E45-N276)

56538  [NCBI]

ETRIIKGYECRPHSQPWQVALFQKTRLLCGATLIAPKWLLTAAHCRKPHYVILLGEHNLEKTDGCEQRRMATESFPHPDFNNSLPNKDHRNDIMLVKMSSPVFFTRAVQPLTLSPHCVAAGTSCLISGWGTTSSPQLRLPHSLRCANVSIIEHKECEKAYPGNITDTMLCASVRKEGKDSCQGDSGGPLVCNGSLQGIISWGQDPCAVTRKPGVYTKVCKYFNWIHEVMRNN

Approximately 35-43 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.