Kallikrein-15 蛋白, Human (HEK293, C-His)

Kallikrein-15 is a kallikrein (KLK) with trypsin-like activity that may promote cancer metastasis. The study found that Kallikrein-15 is only strongly expressed in mature sperm cells and not in immature germ cells. KLK15 may be able to cleave many extracellular matrix (ECM) components, interact with the extracellular matrix (ECM) receptor group, and be associated with tumorigenesis. Kallikrein-15 Protein, Human (HEK293, His) is the recombinant human-derived Kallikrein-15 protein, expressed by HEK293 , with C-His labeled tag.

Kallikrein-15 (KLK15) 是一种激肽释放酶 (KLK)。先前的研究表明 KLK15 可能在前列腺癌中发挥作用。睾丸免疫组织学化学分析表明，KLK15 在成熟精子细胞中强烈表达，但在未成熟生殖细胞中不表达。KLK15 在前列腺上皮基底细胞层的亚细胞定位主要在细胞核中。KLK15 还具有胰蛋白酶样活性，并优先在精氨酸 (R) 之后裂解。KLK15 可能能够裂解许多细胞外基质 (ECM) 成分，其功能与某些 KLK 家族成员相似。KLK15 的酶活性受 pH、阳离子和丝氨酸蛋白酶抑制剂等不同因素的调节。值得注意的是，KLK15 最有可能通过与细胞外基质 (ECM) 受体基团相互作用与肿瘤发生相关，并可能通过这种机制促进转移。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

Q9H2R5-1 (Q17-N256)

55554

QDGDKLLEGDECAPHSQPWQVALYERGRFNCGASLISPHWVLSAAHCQSRFMRVRLGEHNLRKRDGPEQLRTTSRVIPHPRYEARSHRNDIMLLRLVQPARLNPQVRPAVLPTRCPHPGEACVVSGWGLVSHNEPGTAGSPRSQVSLPDTLHCANISIISDTSCDKSYPGRLTNTMVCAGAEGRGAESCEGDSGGPLVCGGILQGIVSWGDVPCDNTTKPGVYTKVCHYLEWIRETMKRN

Approximately 38-45 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.