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  3. Kappa-Casein 蛋白, Human (HEK293, His)

Kappa-Casein 蛋白, Human (HEK293, His)

目录号: HY-P70978
COA 产品使用指南

Kappa-酪蛋白 (CSN3) 是酪蛋白家族的一员,是一种参与多种重要生理过程的细胞外哺乳动物乳蛋白。CSN3 可防止牛奶中的酪蛋白沉淀,提高消化效率,防止新生儿对摄入的蛋白质过敏以及抑制胃部病原体。Kappa-Casein 蛋白, Human (HEK293, His) 是重组的 Kappa 酪蛋白,由 HEK293 表达,带有 C-6*His 标签。Kappa-Casein 蛋白, Human (HEK293, His) 全长 162 个氨基酸,分子量为 28-35 kDa。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

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描述

Kappa-casein (CSN3) is a member of Caseins family, and is an extracellular mammalian milk protein involved in several important physiological processes. CSN3 prevents casein precipitation in milk and increase the efficiency of digestion, prevention of neonate hypersensitivity to ingested proteins, and inhibition of gastric pathogens. Kappa-Casein Protein, Human (HEK293, His) is the recombinant human-derived Kappa-Casein protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Kappa-Casein Protein, Human (HEK293, His) is 162 a.a., with molecular weight of 28-35 kDa.

研究背景

Kappa-酪蛋白 (CSN3) 是酪蛋白家族的一员,是一种参与多种重要生理过程的细胞外哺乳动物乳蛋白。CSN3 稳定胶束形成,防止牛奶中的酪蛋白沉淀。凝乳酶将 CSN3 分裂为不溶性肽 (副 Kappa-酪蛋白) 和水溶性糖巨肽 (GMP)。GMP 负责提高消化效率,预防新生儿对摄入的蛋白质过敏以及抑制胃病原体[1][2]

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

AAH10935.1 (E21-A182)

基因 ID
蛋白结构
N-term
Kappa-Casein (E21-A182)
Accession # AAH10935.1
6*His
C-term
同用名
Kappa-Casein; CSN3; CASK; CSN10; CSNK
氨基酸序列

EVQNQKQPACHENDERPFYQKTAPYVPMYYVPNSYPYYGTNLYQRRPAIAINNPCVPRTYYANPAVVRPHAQIPQRQYLPNSHPPTVVRRPNLHPSFIAIPPKKIQDKIIIPTINTIATVEPTPAPATEPTVDSVVTPEAFSESIITSTPETTTVAVTPPTA

分子量

28-35 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

Kappa-Casein Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Kappa-Casein Protein, Human (HEK293, His)
目录号:
HY-P70978
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