KAT5 蛋白, Human (His)

The KAT5 protein is the catalytic subunit of the NuA4 histone acetyltransferase complex and activates gene transcription through histone acetylation. KAT5 Protein, Human (His) is the recombinant human-derived KAT5 protein, expressed by E. coli , with N-6*His labeled tag. The total length of KAT5 Protein, Human (His) is 511 a.a., with molecular weight of ~62.4 kDa.

KAT5 是 NuA4 组蛋白乙酰转移酶复合物的催化亚基，是通过核小体组蛋白 H2A 和 H4 乙酰化来转录激活特定基因的关键参与者。这种乙酰化修饰核小体-DNA 相互作用，并促进修饰组蛋白与其他蛋白质的相互作用，从而正向调节转录。NuA4 复合物对于生长诱导、生长停滞、细胞凋亡和 DNA 修复等各种细胞过程至关重要，通过抑制 TP53BP1 与染色质的结合，在修复 DNA 双链断裂中发挥直接作用。此外，KAT5 乙酰化参与多种途径的非组蛋白，包括 DNA 修复、昼夜节律调节、自噬、先天抗病毒反应和脂质代谢。它是有丝分裂过程中染色体分离和动粒-微管附着的关键调节因子，催化靶蛋白的乙酰化或巴豆酰化，以确保准确的染色体分离和纺锤体定位。KAT5 的多方面作用使其成为细胞稳态和基因组维护的核心角色。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q92993 (E3-W513)

10524  [NCBI]

EVGEIIEGCRLPVLRRNQDNEDEWPLAEILSVKDISGRKLFYVHYIDFNKRLDEWVTHERLDLKKIQFPKKEAKTPTKNGLPGSRPGSPEREVPASAQASGKTLPIPVQITLRFNLPKEREAIPGGEPDQPLSSSSCLQPNHRSTKRKVEVVSPATPVPSETAPASVFPQNGAARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW

Approximately 62.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.