KGF/FGF-7 蛋白, Human (HEK293, His)

KGF/FGF-7 proteins coordinate embryonic development and regulate basic processes such as cell proliferation and differentiation. Its critical role extends to normal branching morphogenesis. KGF/FGF-7 Protein, Human (HEK293, His) is the recombinant human-derived KGF/FGF-7 protein, expressed by HEK293 , with C-6*His labeled tag.

KGF/FGF-7 蛋白在协调胚胎发育中发挥着至关重要的作用，对细胞增殖和分化等基本过程具有调节控制作用。它的重要贡献延伸到正常分支形态发生的复杂领域，其中 KGF/FGF-7 发挥着关键作用。作为一种对角质形成细胞具有特异性活性的生长因子，它是控制正常上皮细胞增殖的潜在主要旁分泌效应物。该蛋白建立了关键的相互作用，与 FGFBP1 和 FGFR2 形成复合物，并且其与成纤维细胞生长因子 (FGF) 及其受体的结合亲和力通过硫酸乙酰肝素糖胺聚糖增强，在这些分子相互作用中充当关键的辅助受体。

1. The cell proliferation assay using HaCaT cells has an ED₅₀ value of 1.35-10.94 ng/mL.
2. Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED₅₀ this effect is <47 ng/mL, corresponding to a specific activity is >2.13×10⁴ units/mg.
3.Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is 20.27-29.83 ng/mL, corresponding to a specific activity is ≤4.93×10⁴ units/mg.

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  Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED₅₀ this effect is 33.63 ng/mL, corresponding to a specific activity is 2.9735×10⁴ units/mg.

Human

HEK293

C-6*His

P21781-1 (C32-T194)

2252  [NCBI]

CNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT

Approximately 20-28 kDa due to the glycosylation.

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.