Kininogen-1 蛋白, Mouse (HEK293, His)

Together with endopeptidase inhibitor, protease, and signaling receptor binding activities, the Kininogen-1 protein is predicted to play a critical role in antimicrobial response, coagulation regulation, and control of endopeptidase activity.Kininogen-1 is located in the collagen-containing matrix and shows biased expression, particularly in the liver at embryonic (E18) and adult stages (RPKM 646.0 and 179.1).Kininogen-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kininogen-1 protein, expressed by HEK293 , with C-His labeled tag.

Kininogen-1蛋白具有预测的功能，包括半胱氨酸型内肽酶抑制剂活性、蛋白酶结合活性和信号受体结合活性，在各种生物过程中发挥着至关重要的作用。它涉及抗菌体液免疫反应、凝血的负调节和肽链内切酶活性的负调节。Kininogen-1 位于含有胶原蛋白的细胞外基质中，表现出偏向的表达模式，在胚胎 (E18) 和成人阶段 (RPKM 分别为 646.0 和 179.1) 的肝脏中都有显着水平，突显了其在不同肝功能中的重要性。发展阶段。

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (HY-P1759B). The IC₅₀ value is 1.881 nM that incubate at 37℃ for 15 minutes.

Mouse

HEK293

C-His

NP_001095882.1 (E21-S480)

16644  [NCBI]

EEAQEIDCNDEAVFQAVDFSLKQFNPGVKSGNQYMLHRVIEGTKTDGSPTFYSFKYLIKEGNCSAQSGLAWQDCDFKDAEEAATGECTATVGKRENEFFIVTQTCKIAPSKAPILKAYFPCIGCVHAISTDSPDLEPVLKHSIEHFNNNTDHSHLFTLRKVKSAHRQVVAGLNFDITYTIVQTNCSKERFPSLHGDCVALPNGDDGECRGNLFMDINNKIANFSQSCTLYSGDDLVEALPKPCPGCPRDIPVDSPELKEVLGHSIAQLNAENDHPFYYKIDTVKKATSQVVAGTKYVIEFIARETKCSKESNTELAEDCEIKHLGQSLDCNANVYMRPWENKVVPTVKCQALDMTEMARRPPGFSPFRSVTVQETKEGRTDSDFIEDVVATTPPYDTGAHDDLIPDIHVQPDSLSFKLISDFPEATSPKCPGRPWKPASWKDPNTETTEFSDFDLLDALS

70-95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 50 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.