KIRREL3/NEPH2 蛋白, Mouse (HEK293, His)

The KIRREL3/NEPH2 protein plays a critical role in the formation of precise synapses, particularly in hippocampal mossy fiber synapses, where it is responsible for coordinating mossy fiber filopodia. It acts in a homologous manner to stabilize filopodia contacts and facilitate subsequent synapse formation. KIRREL3/NEPH2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived KIRREL3/NEPH2 protein, expressed by HEK293 , with C-His labeled tag. The total length of KIRREL3/NEPH2 Protein, Mouse (HEK293, His) is 514 a.a., with molecular weight of ~60-80 kDa.

KIRREL3/NEPH2 蛋白是一种关键的突触粘附分子，对于目标特异性突触的精确形成至关重要，尤其是在海马苔藓纤维突触中尤为显着。其重要性延伸至苔藓纤维丝状伪足、连接齿状颗粒和 GABA 神经元的关键突触结构的编排。从功能上讲，KIRREL3/NEPH2 可能充当同质粘附分子，促进跨细胞相互作用，并在稳定苔藓纤维丝足接触方面发挥关键作用，从而促进随后的突触形成。在海马体之外，KIRREL3/NEPH2 对于犁鼻感觉神经元轴突的合并至关重要。此外，它可能在基质细胞的造血支持能力中发挥作用，其分泌的细胞外结构域直接支持造血干细胞。作为同源二聚体，KIRREL3/NEPH2 介导对促进细胞粘附至关重要的同源相互作用。此外，它还参与一系列蛋白质-蛋白质相互作用，包括与 NPHS1 形成异二聚体、与 NPHS2/podocin、CASK、MAP1B、MYO16、ATP1B1、SHMT2 和 UFC1 相互作用，强调了其在不同细胞过程中的多方面和不可或缺的作用。

Mouse

HEK293

C-His

Q8BR86 (L22-A535)

67703  [NCBI]

LQKRGCCLVLGYMAKDKFRRMNEGQVYSFSQQPQDQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRSRPARLTVLVPPDDPIILGGPVISLRAGDPLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNIVTFHCSAKANPAVTQYRWAKRGHIIKEASGELYRTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTSEPQSLLVDLGSDAVFSCAWIGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETVNTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQGSEMKSGAGLEAESVPMA

Approximately 60-80 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.