Klotho beta 蛋白, Human (HEK293, His)

Klotho beta protein plays a critical role in the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme controlling bile acid synthesis. Although its glycosidase activity may be inactive, Klotho beta enhances the binding ability of FGFR1 and FGFR4 to FGF21, promoting their interaction. Klotho beta Protein, Human (HEK293, His) is the recombinant human-derived Klotho beta protein, expressed by HEK293 , with C-His labeled tag. The total length of Klotho beta Protein, Human (HEK293, His) is 954 a.a., with molecular weight of 112-140 kDa.

Klotho beta 蛋白通过抑制胆固醇 7-α-羟化酶 (CYP7A1)（胆汁酸合成中的关键酶），在转录调节中发挥重要作用。虽然作为糖苷酶可能没有活性，但 Klotho beta 增强了 FGFR1 和 FGFR4 与 FGF21 的结合能力，表明其参与成纤维细胞生长因子 (FGF) 信号转导。该蛋白直接与 FGF19 和 FGF21 相互作用，强调了它与 FGF 通路关键成分的关联。这些相互作用，特别是与 FGF21 的相互作用，表明在代谢调节中的潜在作用，并强调 Klotho beta 参与调节细胞反应和代谢途径（参考：未提供）。

1.Immobilized Human Beta Klotho at 2 μg/mL (100 μL/Well). Dose response curve for Anti-Beta Klotho Antibody1 with the EC₅₀ is < 80.2 ng/mL determined by ELISA.
2.Immobilized Human Beta Klotho at 2 μg/mL (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody2, hFc Tag with the EC₅₀ of < 89.8 ng/ml determined by ELISA.
3.Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.2198 ng/mL in the presence of 1μg/mL Human FGF-21, corresponding to a specific activity is 4.549×10³ units/mg.

Human

HEK293

C-His

Q86Z14 (M30-T983)

152831  [NCBI]

Approximately 112-140 kDa due to the glycosylation.

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.