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  3. Klotho 蛋白, Human (CHO, His)

Klotho 蛋白, Human (CHO, His)

目录号: HY-P700011
COA 产品使用指南

Klotho 蛋白 对葡萄糖醛酸化类固醇表现出较弱的糖苷酶活性,并且由于缺乏必需的活性位点 Glu 残基,在体内无活性。它通过抑制活性维生素 D 的合成来调节钙和磷的稳态。Klotho 蛋白, Human (CHO, His) 是重组的 Klotho 蛋白,由 CHO 表达,带有 C-10*His 标签。Klotho 蛋白, Human (CHO, His) 全长 948 个氨基酸,分子量为 120-140 kDa。

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规格 价格 是否有货 数量
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5 μg ¥1180 In-stock
10 μg ¥2000 In-stock
50 μg ¥5600 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Klotho Protein exhibits weak glycosidase activity against glucuronidated steroids and is inactive in vivo due to the lack of essential active site Glu residues. It regulates calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Klotho Protein, Human (CHO, His) is the recombinant human-derived Klotho protein, expressed by CHO , with C-10*His labeled tag. The total length of Klotho Protein, Human (CHO, His) is 948 a.a., with molecular weight of 120-140 kDa.

研究背景

Klotho 蛋白对葡萄糖醛酸化类固醇表现出较弱的糖苷酶活性,但由于 239 和 872 位缺少必要的活性位点 Glu 残基,表明其在体内作为糖苷酶无活性。此外,Klotho 可能在钙和磷的调节中发挥作用通过抑制活性维生素 D 的合成来维持体内平衡。作为 FGF23 和 FGFR1 之间特异性相互作用的重要因素,Klotho 有助于关键的信号传导通路。此外,通过裂解膜结合异构体产生的 Klotho 肽被认为可以作为抗衰老循环激素。这种激素有可能通过抑制胰岛素/IGF1 信号传导来延长寿命,使 Klotho 成为一种多功能蛋白质,对代谢调节和衰老过程都有影响。

生物活性

The EC50 as determined by the dose-dependent stimulation of the proliferation of murine NIH/3T3 is less than 1 μg/mL.

种属

Human

表达系统

CHO

标签

C-10*His

蛋白编号

Q9UEF7 (E34-S981)

基因 ID
蛋白结构
N-term
Klotho (E34-S981)
Accession # Q9UEF7
10*His
C-term
同用名
α-Klotho
氨基酸序列

EPGDGAQTWARFSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPGDSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNGSAGVPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDAYGGWANRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGIRGSPRLGYLVAHNLLLAHAKVWHLYNTSFRPTQGGQVSIALSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPVFIDGDYPESMKNNLSSILPDFTESEKKFIKGTADFFALCFGPTLSFQLLDPHMKFRQLESPNLRQLLSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIKLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFYQKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVDTTLSQFTDLNVYLWDVHHSKRLIKVDGVVTKKRKSYCVDFAAIQPQIALLQEMHVTHFRFSLDWALILPLGNQSQVNHTILQYYRCMASELVRVNITPVVALWQPMAPNQGLPRLLARQGAWENPYTALAFAEYARLCFQELGHHVKLWITMNEPYTRNMTYSAGHNLLKAHALAWHVYNEKFRHAQNGKISIALQADWIEPACPFSQKDKEVAERVLEFDIGWLAEPIFGSGDYPWVMRDWLNQRNNFLLPYFTEDEKKLIQGTFDFLALSHYTTILVDSEKEDPIKYNDYLEVQEMTDITWLNSPSQVAVVPWGLRKVLNWLKFKYGDLPMYIISNGIDDGLHAEDDQLRVYYMQNYINEALKAHILDGINLCGYFAYSFNDRTAPRFGLYRYAADQFEPKASMKHYRKIIDSNGFPGPETLERFCPEEFTVCTECSFFHTRKS

分子量

120-140kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, 50% Glycerol, pH7.4.

内毒素含量

<0.1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Klotho Protein, Human (CHO, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Klotho Protein, Human (CHO, His)
目录号:
HY-P700011
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