LASB 蛋白, Pseudomonas aeruginosa (P. pastoris, His)

LASB proteins are multifunctional enzymes that exhibit broad substrate specificity by cleaving host elastin, collagen, IgG, multiple complement components, and endogenous proaminopeptidases. Furthermore, LASB exhibits autocatalytic activity in processing its own propeptide and plays a role in processing the propeptide of chitin-binding protein (cbpD). LASB Protein, Pseudomonas aeruginosa (P. pastoris, His) is the recombinant LASB protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of LASB Protein, Pseudomonas aeruginosa (P. pastoris, His) is 301 a.a., with molecular weight of 35.2 kDa.

LASB (LasA) 蛋白被确定为铜绿假单胞菌的毒力因子，在感染的发病机制中发挥着重要作用。这种钙依赖性金属蛋白酶表现出广泛的底物特异性，可裂解宿主弹性蛋白、胶原蛋白、IgG 和各种补体成分以及内源性氨基肽酶原。LASB 参与其前肽的自催化加工，还加工几丁质结合蛋白原 (cbpD) 的前肽。这些酶活性通过促进组织损伤和免疫逃避来增强细菌的毒力。多样化的底物特异性和自催化过程强调了 LASB 在铜绿假单胞菌致病性中的多方面作用。

Others

P. pastoris

N-6*His

P14756 (A198-L498)

880368  [NCBI]

AEAGGPGGNQKIGKYTYGSDYGPLIVNDRCEMDDGNVITVDMNSSTDDSKTTPFRFACPTNTYKQVNGAYSPLNDAHFFGGVVFKLYRDWFGTSPLTHKLYMKVHYGRSVENAYWDGTAMLFGDGATMFYPLVSLDVAAHEVSHGFTEQNSGLIYRGQSGGMNEAFSDMAGEAAEFYMRGKNDFLIGYDIKKGSGALRYMDQPSRDGRSIDNASQYYNGIDVHHSSGVYNRAFYLLANSPGWDTRKAFEVFVDANRYYWTATSNYNSGACGVIRSAQNRNYSAADVTRAFSTVGVTCPSAL

35.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.