1. 重组蛋白
  2. Receptor Proteins
  3. Layilin/LAYN 蛋白, Human (220a.a, HEK293, His)

Layilin/LAYN 蛋白, Human (220a.a, HEK293, His)

目录号: HY-P73839
COA 产品使用指南

Layilin/LAYN 蛋白作为透明质酸受体并与 NF2、RDX 和 TLN1 相互作用。Layilin/LAYN 蛋白, Human (220a.a, HEK293, His) 是重组的 Layilin/LAYN 蛋白,由 HEK293 表达,带有 C-His 标签。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Layilin/LAYN Protein serves as a receptor for hyaluronate and interacts with NF2, RDX, and TLN1. Layilin/LAYN Protein, Human (220a.a, HEK293, His) is the recombinant human-derived Layilin/LAYN protein, expressed by HEK293 , with C-His labeled tag.

研究背景

Layilin/LAYN 作为透明质酸受体,在细胞与这种细胞外基质成分的相互作用中发挥着至关重要的作用。此外,Layilin/LAYN 与 NF2、RDX 和 TLN1 相互作用,表明其参与这些相互作用蛋白介导的各种细胞过程和信号通路。

生物活性

Measured by its binding ability in a functional ELISA. When LAYN is present at 5 μg/mL, the ED50 for this effect is 22.38 μg/mL.

  • Measured by its binding ability in a functional ELISA.When LAYN is present at 5 μg/mL, the ED50 for this effect is 22.38μg/mL.
种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q6UX15-2/NP_849156.1 (A22-E220)

基因 ID
蛋白结构
N-term
LAYN (A22-E220)
Accession # Q6UX15-2/NP_849156.1
His
C-term
同用名
Layilin; LAYN; LYAN
氨基酸序列

ATGRLLSGQPVCRGGTQRPCYKVIYFHDTSRRLNFEEAKEACRRDGGQLVSIESEDEQKLIEKFIENLLPSDGDFWIGLRRREEKQSNSTACQDLYAWTDGSISQFRNWYVDEPSCGSEVCVVMYHQPSAPAGIGGPYMFQWNDDRCNMKNNFICKYSDEKPAVPSREAEGEETELTTPVLPEETQEEDAKKTFKESRE

分子量

Approximately 27-34 kDa due to the glycosylation

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Layilin/LAYN Protein, Human (220a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Layilin/LAYN Protein, Human (220a.a, HEK293, His)
目录号:
HY-P73839
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