1. 重组蛋白
  2. Receptor Proteins
  3. Layilin/LAYN 蛋白, Rat (HEK293)

Layilin/LAYN 蛋白, Rat (HEK293)

目录号: HY-P74779
COA 产品使用指南 技术支持

Layilin (LAYN) 预计可结合透明质酸,并有望作为膜的组成部分发挥作用。它在脾 (RPKM 32.4) 和肺 (RPKM 17.5) 等组织中的偏向表达强调了 Layilin 在不同生理环境下的不同细胞过程中的潜在作用。Layilin/LAYN 蛋白, Rat (HEK293) 是重组的 Layilin/LAYN 蛋白,由 HEK293 表达,不带标签。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Layilin (LAYN), predicted to bind hyaluronic acid, is expected to function as a membrane integral component. Its biased expression in tissues like the spleen (RPKM 32.4) and lung (RPKM 17.5) underscores Layilin's potential roles in diverse cellular processes across various physiological contexts. Layilin/LAYN Protein, Rat (HEK293) is the recombinant rat-derived Layilin/LAYN protein, expressed by HEK293 , with tag free.

研究背景

Layilin (LAYN) 预计具有透明质酸结合活性,并有望作为细胞膜的组成部分。该蛋白与人 LAYN (layilin) 直系同源,在多种组织中表现出偏向表达,包括脾 (RPKM 32.4) 和肺 (RPKM 17.5),突出显示其在多种细胞过程和生理环境中的潜在参与。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Layilin at 5 µg/mL (100 µL/well) can bind biotinylated hyaluronan. The ED50 for this effect is 7.316 µg/mL.

种属

Rat

表达系统

HEK293

标签

Tag Free

蛋白编号

NP_001178926.1 (H25-E224)

基因 ID
蛋白结构
N-term
LAYN (H25-E224)
Accession # NP_001178926.1
C-term
同用名
Layilin; LAYN; LYAN
氨基酸序列

HLLSGDLDPRGGQRFCWEGTRRPCYRVIYFHDTSQRRNFEEAKEACMKDGGQLASIETADEQRLIENFIESLQASDGDFWIGLKRLEEKQHNSTACQDLYAWTDGSLSQFRNWYMDEPSCESEVCVVMYHQPSAPPGIGGPYMFQWNDDRCNTKKNFICKYSDDKPSTTPSLSPGGEATEPAAPILPEETLEIDTKERRE

分子量

Approximately 30-35 kDa due to the glycosylation.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Layilin/LAYN Protein, Rat (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Layilin/LAYN Protein, Rat (HEK293)
目录号:
HY-P74779
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