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  3. LCN1/Lipocalin-1 蛋白, Human (HEK293, His)

LCN1/Lipocalin-1 蛋白, Human (HEK293, His)

目录号: HY-P75905
COA 产品使用指南

LCN1/Lipocalin-1 蛋白可能参与味觉接收并有助于味觉系统中味觉分子的浓缩和运输。LCN1/Lipocalin-1 蛋白, Human (HEK293, His) 是重组的 LCN1/Lipocalin-1 蛋白,由 HEK293 表达,带有 C-His 标签。LCN1/Lipocalin-1 蛋白, Human (HEK293, His) 全长 158 个氨基酸,分子量约为 ~20 kDa。

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描述

The LCN1/Lipocalin-1 protein may be involved in taste reception and contribute to the concentration and transport of taste molecules in the taste system. LCN1/Lipocalin-1 Protein, Human (HEK293, His) is the recombinant human-derived LCN1/Lipocalin-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of LCN1/Lipocalin-1 Protein, Human (HEK293, His) is 158 a.a., with molecular weight of ~20 kDa.

研究背景

LCN1 (Lipocalin-1) 蛋白与味觉接收有关,可能在味觉系统内味道分子的浓缩和传递中发挥至关重要的作用。LCN1 以其广泛的配体结合能力而闻名,由于其非常宽的配体口袋,它可以与各种配体相互作用,涵盖脂质、类维生素A、大环抗生素利福平和微生物铁载体。该蛋白质主要以单体形式存在,但也可能形成同二聚体。此外,LCN1 与 LMBR1L 相互作用,促进 LCN1 的内吞作用。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P31025 (H19-D176)

基因 ID
蛋白结构
N-term
LCN1 (H19-D176)
Accession # P31025
His
C-term
同用名
Lipocalin-1; Tear lipocalin; Tlc; TP; VEG protein; VEGP
氨基酸序列

HHLLASDEEIQDVSGTWYLKAMTVDREFPEMNLESVTPMTLTTLEGGNLEAKVTMLISGRCQEVKAVLEKTDEPGKYTADGGKHVAYIIRSHVKDHYIFYCEGELHGKPVRGVKLVGRDPKNNLEALEDFEKAAGARGLSTESILIPRQSETCSPGSD

分子量

Approximately 20 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

LCN1/Lipocalin-1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LCN1/Lipocalin-1 Protein, Human (HEK293, His)
目录号:
HY-P75905
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