LDHB 蛋白, Human (His)

The LDHB protein, also known as lactate dehydrogenase B, is an enzyme that plays a crucial role in cellular energy metabolism. References indicate that the LDHB protein effectively catalyzes the simultaneous, stereospecific interconversion of pyruvate and lactate. LDHB Protein, Human (His) is the recombinant human-derived LDHB protein, expressed by E. coli , with N-6*His labeled tag. The total length of LDHB Protein, Human (His) is 334 a.a., with molecular weight of ~37.0 kDa.

LDHB蛋白，也称为乳酸脱氢酶B，是一种在细胞能量代谢中起着至关重要作用的酶。参考文献指出，LDHB 蛋白可有效催化丙酮酸和乳酸同时、立体特异性的相互转化。这种酶活性伴随着 NADH 和 NAD(+) 的相互转化，NADH 和 NAD(+) 是参与氧化还原反应的必需辅酶。LDHB 有助于促进糖酵解产物丙酮酸转化为乳酸，反之亦然。这种相互转化过程对于维持细胞中乳酸和丙酮酸的平衡非常重要，并且对于能量产生和细胞氧化还原状态的调节至关重要。

Measured by its ability to reduce pyruvate to lactate. The specific activity is 101589.5 pmol/min/μg, as measured under the described conditions.

Human

E. coli

N-6*His

P07195 (M1-L334)

3945  [NCBI]

MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL

Approximately 37.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.