1. 重组蛋白
  2. Receptor Proteins
  3. LDLR 蛋白, Cynomolgus (HEK293, His)

LDLR 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P77735
产品使用指南

LDLR 蛋白在胆固醇稳态中发挥着关键作用,与 LDL 结合并通过内吞作用介导细胞摄取。聚集成网格蛋白包被的小坑对于内化至关重要。在微生物感染中,LDLR 作为肝细胞中丙型肝炎病毒的受体,强调其在胆固醇代谢和对病毒感染的细胞反应中的双重功能。LDLR 蛋白, Cynomolgus (HEK293, His) 是重组的 LDLR 蛋白,由 HEK293 表达,带有 C-His 标签。LDLR 蛋白, Cynomolgus (HEK293, His) 全长 767 个氨基酸,分子量为 115-130 kDa。

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描述

LDLR Protein plays a pivotal role in cholesterol homeostasis, binding to LDL and mediating cellular uptake through endocytosis. Clustering into clathrin-coated pits is essential for internalization. In microbial infection, LDLR acts as a receptor for hepatitis C virus in hepatocytes, emphasizing its dual functionality in cholesterol metabolism and the cellular response to viral infections. LDLR Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived LDLR protein, expressed by HEK293 , with C-His labeled tag. The total length of LDLR Protein, Cynomolgus (HEK293, His) is 767 a.a., with molecular weight of 115-130 kDa.

研究背景

LDLR 蛋白通过与低密度脂蛋白 (LDL)(血浆中主要携带胆固醇的脂蛋白)结合,并通过内吞作用促进其细胞摄取,从而成为胆固醇稳态的重要介质。为了实现内化,受体-配体复合物必须首先聚集成网格蛋白包被的凹坑。此外,在微生物感染的情况下,LDLR 作为肝细胞内丙型肝炎病毒的受体,尽管这种相互作用不是通过与病毒蛋白直接结合而发生的。这种双重功能强调了 LDLR 在胆固醇代谢和病毒感染细胞反应中的不同作用。

种属

Cynomolgus

表达系统

HEK293

标签

C-His

蛋白编号

XP_005588053 (A22-G788)

基因 ID
蛋白结构
N-term
LDLR (A22-G788)
Accession # XP_005588053
His
C-term
同用名
LDLR; FHC; LDL R; LDL receptor; LDLCQ2; FH
分子量

115-130 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

LDLR Protein, Cynomolgus (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LDLR Protein, Cynomolgus (HEK293, His)
目录号:
HY-P77735
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