Legumain 蛋白, Mouse (HEK293, C-His)

Legumain proteins can slowly cleave aspartyl bonds, especially under acidic conditions.Legumain Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived Legumain protein, expressed by HEK293 , with C-10*His labeled tag.

Legumain 蛋白对天冬酰胺键的水解表现出严格的特异性。此外，它还表现出缓慢裂解天冬氨酰键的能力，特别是在酸性条件下，进一步扩展了其酶的多功能性。从功能上讲，Legumain 是溶酶体/内体系统内 MHC II 类抗原呈递蛋白质加工不可或缺的一部分。最近的研究结果表明，它还通过促进 Perforin-2 (MPEG1) 的裂解和成熟，从而促进细胞质中的抗原易位，从而在交叉呈递树突状细胞的 MHC I 类抗原呈递中发挥关键作用。此外，Legumain 对于肾近曲小管中正常溶酶体蛋白降解至关重要，并且是内化 EGFR 降解所必需的，这凸显了其在细胞过程和细胞增殖调节中的重要性。

1. Measured in a cell proliferation assay using RAW264.7 cells. The ED₅₀ this effect is 0.07438ng/mL, corresponding to a specific activity is 1.344×10⁷ units/mg.
2. Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >130 pmol/min/µg, as measured under the described conditions.

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  Measured in a cell proliferation assay using RAW264.7 cells.The ED₅₀ for this effect is 0.07438 ng/mL, corresponding to a specific activity is 13444474.3211 units/mg.

Mouse

HEK293

C-His

O89017 (V18-Y435)

19141  [NCBI]

VPVGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIIVMMYDDIANSEENPTPGVVINRPNGTDVYKGVLKDYTGEDVTPENFLAVLRGDAEAVKGKGSGKVLKSGPRDHVFIYFTDHGATGILVFPNDDLHVKDLNKTIRYMYEHKMYQKMVFYIEACESGSMMNHLPDDINVYATTAANPKESSYACYYDEERGTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKSISTMKVMQFQGMKHRASSPISLPPVTHLDLTPSPDVPLTILKRKLLRTNDVKESQNLIGQIQQFLDARHVIEKSVHKIVSLLAGFGETAERHLSERTMLTAHDCYQEAVTHFRTHCFNWHSVTYEHALRYLYVLANLCEAPYPIDRIEMAMDKVCLSHY

Approximately 55-60 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL/Tris, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.