Leptin 蛋白, Human (His)

Leptin is an important energy regulator that regulates appetite, metabolism, and immune responses through its central receptor LEPR. In the hypothalamus, it reduces food intake, affects neuropeptides, and affects bone mass and reproductive function. Leptin Protein, Human (His) is the recombinant human-derived Leptin protein, expressed by E. coli , with N-6*His labeled tag. The total length of Leptin Protein, Human (His) is 146 a.a., with molecular weight of ~17 kDa.

瘦素是能量平衡和体重的关键调节剂，在与其分布于各种组织的受体 LEPR 结合后发挥中枢和外周作用。在下丘脑中，瘦素充当食欲调节因子，通过调节促食欲和促食欲神经肽来减少食物摄入并增加能量消耗。此外，它还影响骨量、下丘脑-垂体-肾上腺激素的分泌和生殖功能。在外周组织中，瘦素增强基础代谢，调节胰腺 β 细胞功能和胰岛素分泌，并对内皮细胞表现出促血管生成作用，影响先天免疫和适应性免疫。在下丘脑弓状核内，瘦素激活 POMC 神经元，在去极化时释放食欲肽，并在超极化时抑制 NPY 神经元，减少食欲肽的释放。除了在饱腹感方面的作用之外，瘦素还通过 PKC 激活和随后的信号通路抑制葡萄糖吸收，从而调节肠道中的营养吸收。瘦素还充当生长因子，影响细胞周期调节和基因表达。此外，它在细胞凋亡、血管生成和免疫反应中发挥作用，在适应性免疫中表现出促炎作用并促进 T helper-1 细胞免疫反应。瘦素的多方面功能凸显了其在维持能量稳态和协调各种生理过程中的复杂参与。

Fully biologically active determined by the dose dependent proliferation of MCF7 cells. The ED₅₀ for this effect is 14.64 ng/mL, corresponding to a specific activity is 6.83×10⁴ units/mg.

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  Fully biologically active determined by the dose dependent proliferation of MCF7 cells. The ED₅₀ for this effect is 14.64 ng/mL, corresponding to a specific activity is 6.83×10⁴ units/mg.

Human

E. coli

N-6*His

P41159 (V22-C167)

3952  [NCBI]

VPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC

Approximately 17 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from after extensive dialysis against 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.