Leptin R/LEPR 蛋白, Human (HEK293, His)

Leptin R/LEPR protein, as the receptor of LEP/leptin, plays a key role in mediating central and peripheral effects through multiple signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, it regulates appetite, influencing orexigenic factors and inhibiting orexigenic neuropeptides. Leptin R/LEPR Protein, Human (HEK293, His) is the recombinant human-derived Leptin R/LEPR protein, expressed by HEK293 , with C-10*His labeled tag.

瘦素 R/LEPR 蛋白作为激素 LEP/瘦素的受体发挥作用，通过激活不同的信号通路（包括 JAK2/STAT3 和 MAPK 级联/FOS）介导其中枢和外周效应，发挥关键作用。在下丘脑中，LEP 作为食欲调节因子，通过影响厌食因子和抑制促食欲神经肽，导致食物摄入量减少和能量消耗增加。此外，它还调节骨量和下丘脑-垂体-肾上腺激素的分泌。在外周，瘦素 R/LEPR 影响基础代谢、生殖功能、胰腺 β 细胞功能、胰岛素分泌、血管生成以及先天和适应性免疫。能量稳态和黑皮质素产生的控制，以及生育力、生长和葡萄糖稳态的调节，涉及不同的信号通路。此外，瘦素 R/LEPR 通过抑制臂旁核神经元参与低血糖的反调节反应，并对 T 淋巴细胞反应具有特定作用，调节初始 T 细胞和记忆 T 细胞的增殖并影响细胞因子的产生。此外，它可能作为 LEP 穿过血脑屏障的转运蛋白，并参与 LEP 内吞作用，尽管不会诱导 STAT3 磷酸化和激活 STAT3。

Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 5 μg/mL (100 μL/well) can bind human Leptin receptor. The ED₅₀ for this effect is 0.2725 μg/mL.

Human

HEK293

C-10*His

P48357-1 (F22-D839)

3953  [NCBI]

FNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPKFNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVFQQIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLEDSPLVPQKGSFQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVIFQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQVKYSENSTTVIREADKIVSATSLLVDSILPGSSYEVQVRGKRLDGPGIWSDWSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMNLAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHHRYAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMKNDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINSIGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMYFIIEWKNLNEDGEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGVGKPKIINSFTQDDIEKHQSD

Approximately 100-180 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 8.0, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.