Leukotriene A4 Hydrolase/LTA4H 蛋白, Human (HEK293, His)

The leukotriene A4 hydrolase/LTA4H protein is a bifunctional zinc metalloenzyme that functions as an epoxide hydrolase to convert LTA4 to the proinflammatory mediator LTB4 and exhibits aminopeptidase activity targeting the N-terminal arginine. In addition to its pro-inflammatory effects, LTA4H also combats inflammation by inactivating the neutrophil attractant Pro-Gly-Pro. Leukotriene A4 Hydrolase/LTA4H Protein, Human (HEK293, His) is the recombinant human-derived Leukotriene A4 Hydrolase/LTA4H protein, expressed by HEK293 , with C-6*His labeled tag.

jADRB1 蛋白是 β-肾上腺素能受体家族的成员，在通过 G 蛋白信号转导介导儿茶酚胺激活腺苷酸环化酶方面发挥着关键作用。它对肾上腺素和去甲肾上腺素具有相似的结合亲和力。此外，ADRB1 蛋白通过 G(s)-α 和 cAMP 介导的信号通路促进 Ras 激活。有趣的是，它还参与调节睡眠/觉醒行为。ADRB1 蛋白通过其 C 端 PDZ 基序直接与 RAPGEF2 相互作用，并进一步与 GOPC、MAGI3 和 DLG4 相互作用。接下来是白三烯 A4 水解酶/LTA4H 蛋白，它是一种双功能锌金属酶，具有环氧化物水解酶 (EH) 和氨肽酶活性。LTA4H 作为环氧化物水解酶，催化 LTA4 转化为促炎介质白三烯 B4 (LTB4)。此外，它还表现出氨肽酶活性，特别针对各种合成三肽的 N 端精氨酸。除了其促炎 EH 活性外，LTA4H 还可以通过其氨肽酶活性，通过灭活中性粒细胞吸引剂 Pro-Gly-Pro (PGP) 来对抗炎症，PGP 是一种由基质金属蛋白酶 9 (MMP9) 和脯氨酰肽酶 (PREPL) 产生的生物活性胶原蛋白片段。此外，LTA4H 参与二十碳五烯酸生物合成 resolvin E1 和 18S-resolvin E1，它们是具有有效抗炎和促溶解作用的脂质介质。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P09960-1 (M1-D611)

4048  [NCBI]

MPEIVDTCSLASPASVCRTKHLHLRCSVDFTRRTLTGTAALTVQSQEDNLRSLVLDTKDLTIEKVVINGQEVKYALGERQSYKGSPMEISLPIALSKNQEIVIEISFETSPKSSALQWLTPEQTSGKEHPYLFSQCQAIHCRAILPCQDTPSVKLTYTAEVSVPKELVALMSAIRDGETPDPEDPSRKIYKFIQKVPIPCYLIALVVGALESRQIGPRTLVWSEKEQVEKSAYEFSETESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGELQNSVKTFGETHPFTKLVVDLTDIDPDVAYSSVPYEKGFALLFYLEQLLGGPEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVDWNAWLYSPGLPPIKPNYDMTLTNACIALSQRWITAKEDDLNSFNATDLKDLSSHQLNEFLAQTLQRAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWEDAIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAVRTYQEHKASMHPVTAMLVGKDLKVD

60-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM MES, 2 mM EDTA, 20% Glycerol, pH 5.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.