LILRB2/CD85d/ILT-4 蛋白, Cynomolgus (HEK293, Fc)

The LILRB2/CD85d/ILT-4 protein is a receptor for class I MHC antigens and can recognize multiple HLA alleles. It crucially downregulates the immune response and builds tolerance. LILRB2/CD85d/ILT-4 Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived LILRB2/CD85d/ILT-4 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of LILRB2/CD85d/ILT-4 Protein, Cynomolgus (HEK293, Fc) is 434 a.a., with molecular weight of 80-90 kDa.

LILRB2/CD85d/ILT-4 蛋白作为 I 类 MHC 抗原的受体，可广泛识别 HLA-A、HLA-B、HLA-C、HLA-G 和 HLA-F 等位基因。它在免疫反应下调和耐受性建立中起着至关重要的作用。具体来说，它识别与 B2M/β-2 微球蛋白和九聚体自肽复合的 HLA-G，导致 1 型调节性 T 细胞和骨髓源性抑制细胞的分化，这对于维持母胎耐受性至关重要。LILRB2 与 CD8A 竞争与 I 类 MHC 抗原的结合，并抑制 FCGR1A 介导的细胞反应，包括蛋白质磷酸化和细胞内钙离子的动员。此外，它在磷酸化时与 PTPN6 相互作用并与 FCGR1A 结合。与肽结合的 HLA-G-B2M 和 HLA-F-B2M 的直接相互作用进一步凸显了其参与免疫调节。

Cynomolgus LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus HLA-G Complex Tetramer, His Tag with an affinity constant of 42.50 nM as determined in SPR assay (Biacore T200).

Cynomolgus

HEK293

C-hFc

XM_015441717 (G24-R457)

102120967  [NCBI]

80-90 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.