LIMS1 蛋白, Mouse (Baculovirus, His)

LIMS1, an adapter protein, crucially links beta-integrins to the actin cytoskeleton and cell surface receptors. Mediating vital cellular processes, it connects with integrin-linked protein kinase 1 (ILK) through its first LIM domain, competing with LIMS2. LIMS1 contributes to the IPP complex, with ILK and LIMS1/LIMS2, vital in cellular signaling. Additionally, it binds to NCK2, linking the complex to cell surface receptors, and interacts with TGFB1I1 through the LIM zinc-binding 5 domain, expanding its molecular associations. LIMS1 Protein, Mouse (Baculovirus, His) is the recombinant mouse-derived LIMS1 protein, expressed by Sf9 insect cells, with N-10*His labeled tag. The total length of LIMS1 Protein, Mouse (Baculovirus, His) is 324 a.a., with molecular weight of ~39.6 kDa.

LIMS1 作为细胞质复合物内的衔接蛋白发挥作用，是 β-整联蛋白和肌动蛋白细胞骨架之间的重要纽带，同时将该复合物连接到细胞表面受体酪氨酸激酶和生长因子受体。它的参与涵盖了基本细胞过程的调节，包括细胞存活、增殖和分化。通过第一个 LIM 结构域，LIMS1 与整合素连接蛋白激酶 1 (ILK) 相互作用，与 LIMS2 竞争性结合。值得注意的是，LIMS1 与 ILK 以及 LIMS1 或 LIMS2 一起构成异三聚体 IPP 复合物，后者在细胞信号传导中发挥着关键作用。此外，LIMS1 与 SH3/SH2 接头 NCK2 建立连接，促进复合物与细胞表面受体的连接。它与 TGFB1I1 的相互作用由 LIM 锌结合 5 结构域介导，进一步扩展了其分子关联网络。

Mouse

Sf9 insect cells

N-10*His

Q99JW4 (A2-K325)

110829  [NCBI]

ANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGDVVSALNKAWCVSCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLSETLGRK

Approximately 39.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.