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  3. Lipocalin-2/NGAL 蛋白, Cynomolgus (HEK293, His)

Lipocalin-2/NGAL 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P701082
产品使用指南

Lipocalin-2/NGAL 是一种多方面的铁转运蛋白,参与多种生物过程,包括细胞凋亡、先天免疫和肾脏发育。Lipocalin-2 通过与铁载体 2,3-二羟基苯甲酸酯结合动态影响细胞铁浓度。Lipocalin-2/NGAL 蛋白, Cynomolgus (HEK293, His) 是重组的 Lipocalin-2/NGAL 蛋白,由 HEK293 表达,带有 C-His 标签。Lipocalin-2/NGAL 蛋白, Cynomolgus (HEK293, His) 全长 178 个氨基酸,分子量为 25-30 kDa。

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描述

Lipocalin-2/NGAL is a multifaceted iron transporter involved in multiple biological processes, including apoptosis, innate immunity, and kidney development. Lipocalin-2 dynamically affects cellular iron concentration through binding to the siderophore 2,3-dihydroxybenzoate. Lipocalin-2/NGAL Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Lipocalin-2/NGAL protein, expressed by HEK293 , with C-His labeled tag. The total length of Lipocalin-2/NGAL Protein, Cynomolgus (HEK293, His) is 178 a.a., with molecular weight of 25-30 kDa.

研究背景

Lipocalin-2/NGAL 是一种多方面的铁转运蛋白,参与多种生物过程,包括细胞凋亡、先天免疫和肾脏发育。通过与铁载体 2,3-二羟基苯甲酸 (2,3-DHBA) 的结合,Lipocalin-2 结合并穿梭铁,动态影响基于 Holo-24p3(铁结合)或 apo-24p3(铁)的细胞铁浓度。-免费)表格。与 SLC22A17 受体的相互作用介导铁释放或螯合,精细调节细胞内铁水平。在白细胞介素 3 (IL3) 剥夺引发的细胞凋亡中,含铁形式可防止细胞凋亡,而无铁形式则诱导 BCL2L11/BIM 表达,导致程序性细胞死亡。Lipocalin-2 还通过隔离细菌铁载体(例如肠杆菌素)来促进先天免疫,并表现出结合结核分枝杆菌铁载体的能力。在结构上,Lipocalin-2 以单体、同二聚体(二硫键连接)形式存在,并与 MMP9 形成异二聚体(二硫键连接)。这些不同的相互作用强调了 Lipocalin-2 在协调关键细胞和免疫反应方面的多功能性。

种属

Cynomolgus

表达系统

HEK293

标签

C-His

蛋白编号

XP_005580845.2 (Q21-G198)

基因 ID

/

蛋白结构
N-term
NGAL (Q21-G198)
Accession # XP_005580845.2
His
C-term
同用名
NGAL; Lipocalin-2; Oncogene 24p3; p25; Siderocalin LCN2; MSFI
分子量

25-30 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Lipocalin-2/NGAL Protein, Cynomolgus (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Lipocalin-2/NGAL Protein, Cynomolgus (HEK293, His)
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HY-P701082
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