Lipocalin-2/NGAL 蛋白, Mouse (HEK293, C-His)

Lipocalin-2/NGAL Protein, Mouse (HEK293, His) is an 20-28 kDa NGAL protein with a His-flag, which is expressed in HEK293 cells. NGAL is an antibacterial factor of natural immunity, and an acute-phase protein^[1].

NGAL 是一种 25 kDa 的蛋白质，属于脂质运载蛋白超家族，最初被认为是天然免疫的抗菌因子，也是一种急性期蛋白。
NGAL 最初发现于活化的中性粒细胞和其他细胞（如肾小管）中。细胞可能会产生 NGAL 以应对各种损伤。ipocalin-2 (LCN2/NGAL) 参与多种过程，包括细胞分化、增殖、存活和形态发生。NGAL 在肿瘤中具有抗肿瘤和抗转移作用，例如结肠、卵巢和胰腺^[1]。

Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3] that Incubate at room temperature for 10 minutes. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in NGAL. ≤1.852 μM of Fe(DHBA)3 can be bound.

Mouse

HEK293

C-6*His

P11672 (Q21-N200)

16819  [NCBI]

QDSTQNLIPAPSLLTVPLQPDFRSDQFRGRWYVVGLAGNAVQKKTEGSFTMYSTIYELQENNSYNVTSILVRDQDQGCRYWIRTFVPSSRAGQFTLGNMHRYPQVQSYNVQVATTDYNQFAMVFFRKTSENKQYFKITLYGRTKELSPELKERFTRFAKSLGLKDDNIIFSVPTDQCIDN

Approximately 20-28 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Histidine-HCl, 8% Trehalose, 2% Glycine, 50 mM NaCl, 0.05% Tween 80, pH 6.5 or PBS, pH 7.4 or 20 mM MES, 150 mM NaCl, pH 5.5, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.