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  3. Lipocalin-2/NGAL 蛋白, Mouse (HEK293, Fc)

Lipocalin-2/NGAL 蛋白, Mouse (HEK293, Fc)

目录号: HY-P78329
产品使用指南

Lipocalin-2/NGAL 是一种铁转运蛋白,与 2,3-DHBA 相互作用并调节细胞铁水平。它充当铁的传送器或提取器,具体取决于细胞环境。Lipocalin-2/NGAL可以增加或减少细胞内铁浓度,影响细胞凋亡和先天免疫。它还可以结合结核分枝杆菌的铁载体,并以单体、同二聚体或与 MMP9 的异二聚体形式存在。Lipocalin-2/NGAL 蛋白, Mouse (HEK293, Fc) 是重组的 Lipocalin-2/NGAL 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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描述

Lipocalin-2/NGAL, an iron-trafficking protein, interacts with 2,3-DHBA and modulates cellular iron levels.It acts as a conveyor or extractor of iron, depending on the cellular context.Lipocalin-2/NGAL can increase intracellular iron concentration or reduce it, affecting apoptosis and innate immunity.It can also bind siderophores from M.tuberculosis and exist as a monomer, homodimer, or heterodimer with MMP9.Lipocalin-2/NGAL Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Lipocalin-2/NGAL protein, expressed by HEK293 , with C-hFc labeled tag.

研究背景

Lipocalin-2/NGAL 是一种铁转运蛋白,参与多种生物过程,包括细胞凋亡、先天免疫和肾脏发育。通过与铁载体 2,3-二羟基苯甲酸 (2,3-DHBA)(让人想起细菌肠杆菌素)的相互作用,Lipocalin-2/NGAL 可调节细胞铁水平,根据细胞环境充当铁的输送器或提取器。铁结合形式 (holo-24p3) 在与 SLC22A17 (24p3R) 受体结合后被内化,释放铁并提高细胞内铁浓度。相反,无铁形式 (apo-24p3) 在与 SLC22A17 (24p3R) 结合后,与细胞内铁载体结合,导致铁螯合和随后的细胞外铁转移,从而降低细胞内铁水平。在白介素 3 (IL3) 剥夺诱导的细胞凋亡领域,铁负载形式通过增加细胞内铁浓度来避免细胞凋亡,而无铁形式通过降低细胞内铁水平来促进细胞凋亡,诱导促凋亡蛋白 BCL2L11 的表达/BIM。Lipocalin-2/NGAL 还通过螯合与微生物铁载体(如肠杆菌素)结合的铁来抑制细菌增殖,从而在先天免疫中发挥着至关重要的作用。此外,它还具有结合结核分枝杆菌铁载体的能力。Lipocalin-2/NGAL可以单体、通过二硫键连接的同二聚体或与MMP9的异二聚体的形式存在,进一步扩展了其功能的多功能性。

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

P11672 (Q21-N200)

基因 ID
蛋白结构
N-term
NGAL (Q21-N200)
Accession # P11672
hFc
C-term
同用名
NGAL; Lipocalin-2; Oncogene 24p3; p25; Siderocalin LCN2; MSFI
分子量

50-60 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Lipocalin-2/NGAL Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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