1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. lipoxygenase 蛋白, Human (Flag, His, CL7)

lipoxygenase 蛋白, Human (Flag, His, CL7)

目录号: HY-P703511
产品使用指南

脂氧合酶蛋白催化氧介导的亚油酸转化为9HPOD,并自发分解为9HOD。它将亚麻酸转化为 13HPOD,13HPOD 又分解为 13HOD。lipoxygenase 蛋白, Human (Flag, His, CL7) 是重组的 脂氧合酶,由 E. coli 表达,带有 CL7, His, Flag 标签。lipoxygenase 蛋白, Human (Flag, His, CL7)

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描述

The lipoxygenase protein catalyzes the oxygen-mediated conversion of linoleic acid to 9HPOD and spontaneously decomposes it into 9HOD. It converts linolenic acid into 13HPOD, which breaks down into 13HOD. lipoxygenase Protein, Human (Flag, His, CL7) is the recombinant human-derived lipoxygenase, expressed by E. coli , with CL7, His, Flag labeled tag. ,

研究背景

在氧气存在下,脂氧合酶蛋白催化亚油酸酯转化为 (9S)-氢过氧-10,12-十八烯酸酯 (9HPOD),随后自发分解为相应的 9-羟基-10,12-十八烯酸酯 (9HOD)。它还将亚麻酸酯转化为其氢过氧衍生物,13-氢过氧-9,11-十八碳烯酸酯(13HPOD),后者自发分解为相应的13-羟基-9,11-十八碳烯酸酯(13HOD)。此外,该酶对油酸表现出活性(尽管程度较低),将其转化为 (10S)-氢过氧-8E-十八烯酸,其自发分解形成相应的 10-羟基-8E-十八烯酸。值得注意的是,脂氧合酶对花生四烯酸的活性极低。这种酶的多功能性凸显了其在介导多种脂肪酸氧合反应中的作用,有助于与脂质信号传导和炎症相关的复杂代谢途径。

种属

Human

表达系统

E. coli

标签

CL7;His;Flag

蛋白编号

Q8RNT4 (M1-I685)

基因 ID

/

同用名
Linoleate 9/13-lipoxygenase; Oleate 10S-lipoxygenase; 1.13.11.77; lox; Pseudomonas aeruginosa; Dioxygenase; Oxidoreductase; 1.13.11.12
分子量

Approximately 91.3 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

lipoxygenase Protein, Human (Flag, His, CL7) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
lipoxygenase Protein, Human (Flag, His, CL7)
目录号:
HY-P703511
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