1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. LMW-PTP/ACP1 蛋白, Human (C-His)

LMW-PTP/ACP1 蛋白, Human (C-His)

目录号: HY-P70327A
COA 产品使用指南

LMW-PTP/ACP1 是一种磷酸酶,作用于酪氨酸磷酸化蛋白、低分子量芳基磷酸酯以及天然和合成的酰基磷酸酯。值得注意的是,异构体 1 和异构体 2 之间存在底物特异性差异,其中异构体 2 缺乏磷酸酶活性。LMW-PTP/ACP1 蛋白, Human (C-His) 是重组的 LMW-PTP/ACP1 蛋白,由 E. coli 表达,带有 C-6*His 标签。LMW-PTP/ACP1 蛋白, Human (C-His) 全长 157 个氨基酸,分子量约为 ~18.0 kDa。

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5 μg ¥670 In-stock
10 μg ¥1140 In-stock
50 μg ¥3200 询价
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

LMW-PTP/ACP1, a phosphatase, acts on tyrosine phosphorylated proteins, low-molecular-weight aryl phosphates, and natural and synthetic acyl phosphates. Notably, there are substrate specificity differences between isoform 1 and isoform 2, with isoform 2 lacking phosphatase activity. LMW-PTP/ACP1 Protein, Human (C-His) is the recombinant human-derived LMW-PTP/ACP1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of LMW-PTP/ACP1 Protein, Human (C-His) is 157 a.a., with molecular weight of ~18.0 kDa.

研究背景

LMW-PTP(低分子量蛋白酪氨酸磷酸酶)也称为 ACP1,作为磷酸酶作用于酪氨酸磷酸化蛋白、低分子量芳基磷酸酯以及天然和合成的酰基磷酸酯。值得注意的是,异构体 1 和异构体 2 之间的底物特异性存在差异。需要强调的是,异构体 2 不具有磷酸酶活性。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

P24666-2 (A2-H158)

基因 ID

52  [NCBI]

蛋白结构
N-term
ACP1 (A2-H158)
Accession # P24666-2
6*His
C-term
同用名
rHuLow molecular weight phosphotyrosine protein phosphatase/LMW-PTP, His; Low Molecular Weight Phosphotyrosine Protein Phosphatase; LMW-PTP; LMW-PTPase; Adipocyte Acid Phosphatase; Low Molecular Weight Cytosolic Acid Phosphatase; Red Cell Acid Phosphatase 1; ACP1
氨基酸序列

AEQATKSVLFVCLGNICRSPIAEAVFRKLVTDQNISENWVIDSGAVSDWNVGRSPDPRAVSCLRNHGIHTAHKARQITKEDFATFDYILCMDESNLRDLNRKSNQVKTCKAKIELLGSYDPQKQLIIEDPYYGNDSDFETVYQQCVRCCRAFLEKAH

分子量

Approximately 18.0 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

LMW-PTP/ACP1 Protein, Human (C-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LMW-PTP/ACP1 Protein, Human (C-His)
目录号:
HY-P70327A
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