LOXL2 蛋白, Mouse (HEK293, His)

LOXL2 proteins play multifaceted roles in cellular processes by mediating post-translational oxidative deamination of lysine residues, leading to lysine formation. As a transcriptional corepressor, it specifically deaminates histone H3 trimethylated at "Lys-4" (H3K4me3), which is associated with transcriptional activation. LOXL2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived LOXL2 protein, expressed by HEK293 , with C-His labeled tag.

LOXL2 蛋白在细胞过程中发挥多方面的作用，介导目标蛋白上赖氨酸残基的翻译后氧化脱氨，导致脱氨赖氨酸 (allysine) 的形成。作为转录辅阻遏物，LOXL2 特异性介导组蛋白 H3 (H3K4me3) 三甲基化“Lys-4”的脱氨基作用，组蛋白 H3 是与表观遗传转录激活相关的标签。有趣的是，当 LOXL2 在“Lys-9”(H3K9me3) 或“Lys-27”(H3K27me3) 上三甲基化，或当“Lys-4”单甲基化 (H3K4me1) 或二甲基化 (H3K4me2) 时，LOXL2 对组蛋白 H3 没有活性。此外，LOXL2 介导甲基化 TAF10（转录因子 IID (TFIID) 复合物的成员）的脱氨基作用，诱导 TAF10 从启动子释放并抑制 TFIID 依赖性转录。这一过程导致对胚胎干细胞多能性至关重要的基因的转录抑制。LOXL2 还通过与 SNAI1 相互作用并参与 E-钙粘蛋白的抑制而参与上皮间质转化 (EMT)。此外，LOXL2 可能通过 IV 型胶原蛋白支架参与血管生成调节，并充当软骨细胞分化的调节剂，展示了其在细胞和发育过程中多样化和复杂的作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

HEK293

C-His

P58022-1 (Q26-Q776)

94352  [NCBI]

QYEGWPYQLQYPEYFQQPAPEHHQRQVPSDVVKIQVRLAGQKRKHNEGRVEVYYEGQWGTVCDDDFSIHAAHVVCRQVGYVEAKSWAASSSYGPGEGPIWLDNIYCTGKESTLASCSSNGWGVTDCKHTEDVGVVCSEKRIPGFKFDNSLINQIESLNIQVEDIRIRPILSAFRHRKPVTEGYVEVKEGKAWKQICNKHWTAKNSHVVCGMFGFPAEKTYNPKAYKTFASRRKLRYWKFSMNCTGTEAHISSCKLGPSVTRDPVKNATCENGQPAVVSCVPSQIFSPDGPSRFRKAYKPEQPLVRLRGGAQVGEGRVEVLKNGEWGTICDDKWDLVSASVVCRELGFGTAKEAITGSRLGQGIGPIHLNEVQCTGTEKSIIDCKFNTESQGCNHEEDAGVRCNIPIMGFQKKVRLNGGRNPYEGRVEVLTERNGSLVWGTVCGQNWGIVEAMVVCRQLGLGFASNAFQETWYWHGNIFANNVVMSGVKCSGTELSLAHCRHDEEVACPEGGVRFGAGVACSETAPDLVLNAEIVQQTAYLEDRPMSLLQCAMEENCLSASAVHTDPTRGHRRLLRFSSQIHNNGQSDFRPKNGRHAWIWHDCHRHYHSMEVFTYYDLLSLNGTKVAEGHKASFCLEDTECEGDIQKSYECANFGEQGITMGCWDMYRHDIDCQWIDITDVPPGDYLFQVVINPNYEVPESDFSNNIMKCRSRYDGYRIWMYNCHVGGAFSEETEQKFEHFSGLLNNQLSVQ

90-100 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.