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  3. LRRC52 蛋白, Human (HEK293, His)

LRRC52 蛋白, Human (HEK293, His)

目录号: HY-P77991
产品使用指南

LRRC52 蛋白与 BK 通道相关,可至关重要地调节其门控特性。它会引起激活电压显着的超极化转变,尤其是在没有钙的情况下。作为 KCNU1 辅助蛋白,LRRC52 可能影响 KCNU1 门控。LRRC52 与 KCNU1 的相互作用对于稳定性至关重要,可能会改变通道门控。LRRC52 还与 KCNMA1 相互作用,进一步表明其在调节 BK 通道活性中的作用。LRRC52 蛋白, Human (HEK293, His) 是重组的 LRRC52 蛋白,由 HEK293 表达,带有 C-His 标签。LRRC52 蛋白, Human (HEK293, His) 全长 221 个氨基酸,分子量为 35-48 kDa。

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描述

LRRC52 Protein, associated with the BK channel, crucially modulates its gating properties. It induces a significant hyperpolarizing shift in activation voltage, especially without calcium. As a KCNU1 auxiliary protein, LRRC52 may influence KCNU1 gating. LRRC52's interaction with KCNU1 is essential for stability, potentially altering channel gating. LRRC52 also interacts with KCNMA1, further indicating its role in regulating BK channel activity. LRRC52 Protein, Human (HEK293, His) is the recombinant human-derived LRRC52 protein, expressed by HEK293, with C-His labeled tag. The total length of LRRC52 Protein, Human (HEK293, His) is 221 a.a., with molecular weight of 35-48 kDa.

研究背景

LRRC52 是一种与大电导、电压和钙激活钾通道 (BK α) 相关的辅助蛋白,在调节 BK 通道的门控特性中发挥着至关重要的作用。它引起超极化方向激活的电压依赖性的显着变化,特别是在没有钙的情况下。作为 KCNU1 通道辅助蛋白,LRRC52 也可能影响 KCNU1 的门控特性。LRRC52 和 KCNU1 之间的相互作用被认为对于 LRRC52 稳定性至关重要,并且可能有助于通道门控特性的改变。此外,LRRC52 与 KCNMA1 相互作用,进一步暗示其参与 BK 通道活性的调节。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q8N7C0 (S24-D244)

基因 ID

440699  [NCBI]

蛋白结构
N-term
LRRC52 (S24-D244)
Accession # Q8N7C0
His
C-term
同用名
LRRC52; FLJ25811
分子量

35-48 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

LRRC52 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LRRC52 Protein, Human (HEK293, His)
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