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  3. LTC4S 蛋白, Human (sf9, His)

LTC4S 蛋白, Human (sf9, His)

目录号: HY-P75919
产品使用指南

LTC4S 蛋白催化白三烯 A4 与还原型谷胱甘肽 (GSH) 特异性结合,生成白三烯 C4。LTC4S 蛋白, Human (sf9, His) 是重组的 LTC4S 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。LTC4S 蛋白, Human (sf9, His) 全长 150 个氨基酸,分子量约为 ~17 kDa。

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描述

LTC4S protein catalyzes the specific binding of leukotriene A4 and reduced glutathione (GSH) to generate leukotriene C4. LTC4S Protein, Human (sf9, His) is the recombinant human-derived LTC4S protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of LTC4S Protein, Human (sf9, His) is 150 a.a., with molecular weight of ~17 kDa.

研究背景

LTC4S 通过催化白三烯 A4 与还原型谷胱甘肽 (GSH) 的结合,产生具有显着特异性的生物活性脂质介质白三烯 C4,从而在细胞过程中发挥着至关重要的作用。这种酶活性对于炎症和免疫反应的调节至关重要。此外,LTC4S 通过促进谷胱甘肽基团从谷胱甘肽 (GSH) 转移到 13(S),14(S)-环氧二十二碳六烯酸,从而在组织再生 1 (MCTR1) 中形成 maresin 缀合物,从而展现出多功能性。值得注意的是,MCTR1 具有有效的抗炎和促消退作用,强调了 LTC4S 在介导脂质代谢和炎症方面的多方面性质。

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

Q16873 (M1-A150)

基因 ID
蛋白结构
N-term
LTC4S (M1-A150)
Accession # Q16873
His
C-term
同用名
Leukotriene C4 synthase; LTC4 synthase; LTC4S
分子量

Approximately 17 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

LTC4S Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LTC4S Protein, Human (sf9, His)
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HY-P75919
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